This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hawser, S. P.
Right arrow Articles by Islam, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hawser, S. P.
Right arrow Articles by Islam, K.

 Previous Article  |  Next Article 

Infect. Immun., Jan 1998, 140-144, Vol 66, No. 1
Copyright © 1998, American Society for Microbiology

Binding of Candida albicans to immobilized amino acids and bovine serum albumin

SP Hawser and K Islam
Lepetit Research Center, Gerenzano (VA), Italy.

In this study, we examined the binding of Candida albicans synchronized yeast-phase cells to plastic, immobilized amino acids and bovine serum albumin (BSA) and quantified the binding by using an XTT tetrazolium salt assay and absorbance determination. Our results show that C. albicans binds efficiently and specifically to several nonpolar aliphatic amino acids and positively charged amino acids and to BSA immobilized on tissue culture plastic but not to polar uncharged, negatively charged, or aromatic amino acids. Adhesion of yeasts to immobilized amino acids was not affected by preincubation of cells with BSA, whereas binding to immobilized BSA was affected by preincubation of yeasts with alanine, proline, and leucine but not by arginine or lysine. The ability to distinguish the chirality of these amino acids was also examined by using both the D and L amino acid configurations, and the results show that C. albicans yeasts recognize only the L configuration of these amino acids. The observations that C. albicans specifically binds to certain amino acids indicate that these amino acids may prove useful tools for studying the binding interactions of C. albicans yeasts with host proteins such as components of the extracellular matrix.


This article has been cited by other articles:

  • Becker, K., Almasri, A. S., von Eiff, C., Peters, G., Heilmann, C., Fegeler, W. (2007). Systematic Survey of Nonspecific Agglutination by Candida spp. in Latex Assays. J. Clin. Microbiol. 45: 1315-1318 [Abstract] [Full Text]  
  • Klotz, S. A., Gaur, N. K., Lake, D. F., Chan, V., Rauceo, J., Lipke, P. N. (2004). Degenerate Peptide Recognition by Candida albicans Adhesins Als5p and Als1p. Infect. Immun. 72: 2029-2034 [Abstract] [Full Text]  
  • Kuhn, D. M., Balkis, M., Chandra, J., Mukherjee, P. K., Ghannoum, M. A. (2003). Uses and Limitations of the XTT Assay in Studies of Candida Growth and Metabolism. J. Clin. Microbiol. 41: 506-508 [Abstract] [Full Text]  
  • Kuhn, D. M., Chandra, J., Mukherjee, P. K., Ghannoum, M. A. (2002). Comparison of Biofilms Formed by Candidaalbicans and Candidaparapsilosis on Bioprosthetic Surfaces. Infect. Immun. 70: 878-888 [Abstract] [Full Text]  
  • Islam, K., Hawser, S. P. (1999). Effect of antifungal agents on the binding of Candida albicans to immobilized amino acids and bovine serum albumin. J Antimicrob Chemother 43: 583-587 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»