This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by St. Geme, J. W. Articles by Barenkamp, S. J. Search for Related Content PubMed PubMed Citation Articles by St. Geme, J. W., III Articles by Barenkamp, S. J.

 Previous Article  |  Next Article 

Infect. Immun., Jan 1998, 364-368, Vol 66, No. 1
Copyright © 1998, American Society for Microbiology

Prevalence and distribution of the hmw and hia genes and the HMW and Hia adhesins among genetically diverse strains of nontypeable Haemophilus influenzae

JW St. Geme 3rd, VV Kumar, D Cutter and SJ Barenkamp
Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, and St. Louis Children's Hospital, Missouri 63110, USA. stgeme@borcim.wustl.edu

Nontypeable Haemophilus influenzae is a common cause of human disease and initiates infection by colonizing the upper respiratory tract. In previous work we identified high-molecular-weight adhesins referred to as HMW1 and HMW2, expressed by nontypeable strain 12, and determined that most strains of nontypeable H. influenzae express one or two antigenically related proteins. More recently, we determined that some strains lack HMW1- and HMW2-like proteins and instead express an adhesin called Hia. In the present study, we determined the prevalence and distribution of the hmw and hia genes in a collection of 59 nontypeable strains previously characterized in terms of genetic relatedness. Based on Southern analysis, 47 strains contained sequences homologous to the hmw1 and hmw2 genes and nine strains contained homologs to hia. No strain harbored both hmw and hia, and three strains harbored neither. Although the hmw and hia genes failed to define distinct genetic divisions, the hmw-deficient strains formed small clusters or lineages within the larger population structure. Additional analysis established that the IS1016 insertion element was uniformly absent from strains containing hmw sequences but was present in two- thirds of the hmw-deficient strains. As IS1016 is associated with the capsule locus (cap) in most encapsulated strains of H. influenzae, we speculate that hmw-deficient nontypeable strains evolved more recently from an encapsulated ancestor.

This article has been cited by other articles:

• Sheets, A. J., Grass, S. A., Miller, S. E., St. Geme, J. W. III (2008). Identification of a Novel Trimeric Autotransporter Adhesin in the Cryptic Genospecies of Haemophilus. J. Bacteriol. 190: 4313-4320 [Abstract] [Full Text]  
• Erwin, A. L., Sandstedt, S. A., Bonthuis, P. J., Geelhood, J. L., Nelson, K. L., Unrath, W. C. T., Diggle, M. A., Theodore, M. J., Pleatman, C. R., Mothershed, E. A., Sacchi, C. T., Mayer, L. W., Gilsdorf, J. R., Smith, A. L. (2008). Analysis of Genetic Relatedness of Haemophilus influenzae Isolates by Multilocus Sequence Typing. J. Bacteriol. 190: 1473-1483 [Abstract] [Full Text]  
• Yeo, H.-J., Yokoyama, T., Walkiewicz, K., Kim, Y., Grass, S., Geme, J. W. St. III (2007). The Structure of the Haemophilus influenzae HMW1 Pro-piece Reveals a Structural Domain Essential for Bacterial Two-partner Secretion. J. Biol. Chem. 282: 31076-31084 [Abstract] [Full Text]  
• Satola, S. W., Collins, J. T., Napier, R., Farley, M. M. (2007). Capsule Gene Analysis of Invasive Haemophilus influenzae: Accuracy of Serotyping and Prevalence of IS1016 among Nontypeable Isolates. J. Clin. Microbiol. 45: 3230-3238 [Abstract] [Full Text]  
• Juliao, P. C., Marrs, C. F., Xie, J., Gilsdorf, J. R. (2007). Histidine Auxotrophy in Commensal and Disease-Causing Nontypeable Haemophilus influenzae. J. Bacteriol. 189: 4994-5001 [Abstract] [Full Text]  
• Balder, R., Hassel, J., Lipski, S., Lafontaine, E. R. (2007). Moraxella catarrhalis Strain O35E Expresses Two Filamentous Hemagglutinin-Like Proteins That Mediate Adherence to Human Epithelial Cells. Infect. Immun. 75: 2765-2775 [Abstract] [Full Text]  
• Petris, C. K., Golomb, M., Phillips, T. E. (2007). Bacterial Transcytosis across Conjunctival M Cells. IOVS 48: 2172-2177 [Abstract] [Full Text]  
• Winter, L. E., Barenkamp, S. J. (2006). Antibodies Specific for the High-Molecular-Weight Adhesion Proteins of Nontypeable Haemophilus influenzae Are Opsonophagocytic for both Homologous and Heterologous Strains. CVI 13: 1333-1342 [Abstract] [Full Text]  
• Cotter, S. E., Surana, N. K., Grass, S., St. Geme, J. W. III (2006). Trimeric autotransporters require trimerization of the passenger domain for stability and adhesive activity.. J. Bacteriol. 188: 5400-5407 [Abstract] [Full Text]  
• Fernaays, M. M., Lesse, A. J., Sethi, S., Cai, X., Murphy, T. F. (2006). Differential Genome Contents of Nontypeable Haemophilus influenzae Strains from Adults with Chronic Obstructive Pulmonary Disease.. Infect. Immun. 74: 3366-3374 [Abstract] [Full Text]  
• Giufre, M., Muscillo, M., Spigaglia, P., Cardines, R., Mastrantonio, P., Cerquetti, M. (2006). Conservation and Diversity of HMW1 and HMW2 Adhesin Binding Domains among Invasive Nontypeable Haemophilus influenzae Isolates. Infect. Immun. 74: 1161-1170 [Abstract] [Full Text]  
• Erwin, A. L., Nelson, K. L., Mhlanga-Mutangadura, T., Bonthuis, P. J., Geelhood, J. L., Morlin, G., Unrath, W. C. T., Campos, J., Crook, D. W., Farley, M. M., Henderson, F. W., Jacobs, R. F., Muhlemann, K., Satola, S. W., van Alphen, L., Golomb, M., Smith, A. L. (2005). Characterization of Genetic and Phenotypic Diversity of Invasive Nontypeable Haemophilus influenzae. Infect. Immun. 73: 5853-5863 [Abstract] [Full Text]  
• Cotter, S. E., Yeo, H.-J., Juehne, T., St. Geme, J. W. III (2005). Architecture and Adhesive Activity of the Haemophilus influenzae Hsf Adhesin. J. Bacteriol. 187: 4656-4664 [Abstract] [Full Text]  
• Ecevit, I. Z., McCrea, K. W., Marrs, C. F., Gilsdorf, J. R. (2005). Identification of New hmwA Alleles from Nontypeable Haemophilus influenzae. Infect. Immun. 73: 1221-1225 [Abstract] [Full Text]  
• Ecevit, I. Z., McCrea, K. W., Pettigrew, M. M., Sen, A., Marrs, C. F., Gilsdorf, J. R. (2004). Prevalence of the hifBC, hmw1A, hmw2A, hmwC, and hia Genes in Haemophilus influenzae Isolates. J. Clin. Microbiol. 42: 3065-3072 [Abstract] [Full Text]  
• Buscher, A. Z., Burmeister, K., Barenkamp, S. J., St. Geme, J. W. III (2004). Evolutionary and Functional Relationships among the Nontypeable Haemophilus influenzae HMW Family of Adhesins. J. Bacteriol. 186: 4209-4217 [Abstract] [Full Text]  
• Surana, N. K., Cutter, D., Barenkamp, S. J., St. Geme, J. W. III (2004). The Haemophilus influenzae Hia Autotransporter Contains an Unusually Short Trimeric Translocator Domain. J. Biol. Chem. 279: 14679-14685 [Abstract] [Full Text]  
• Winter, L. E., Barenkamp, S. J. (2003). Human Antibodies Specific for the High-Molecular-Weight Adhesion Proteins of Nontypeable Haemophilus influenzae Mediate Opsonophagocytic Activity. Infect. Immun. 71: 6884-6891 [Abstract] [Full Text]  
• O'Neill, J. M., St. Geme III, J. W., Cutter, D., Adderson, E. E., Anyanwu, J., Jacobs, R. F., Schutze, G. E. (2003). Invasive Disease Due to Nontypeable Haemophilus influenzae among Children in Arkansas. J. Clin. Microbiol. 41: 3064-3069 [Abstract] [Full Text]  
• Rodriguez, C. A., Avadhanula, V., Buscher, A., Smith, A. L., St. Geme III, J. W., Adderson, E. E. (2003). Prevalence and Distribution of Adhesins in Invasive Non-Type b Encapsulated Haemophilus influenzae. Infect. Immun. 71: 1635-1642 [Abstract] [Full Text]  
• Swords, W. E., Chance, D. L., Cohn, L. A., Shao, J., Apicella, M. A., Smith, A. L. (2002). Acylation of the Lipooligosaccharide of Haemophilus influenzae and Colonization: an htrB Mutation Diminishes the Colonization of Human Airway Epithelial Cells. Infect. Immun. 70: 4661-4668 [Abstract] [Full Text]  
• Pettigrew, M. M., Foxman, B., Marrs, C. F., Gilsdorf, J. R. (2002). Identification of the Lipooligosaccharide Biosynthesis Gene lic2B as a Putative Virulence Factor in Strains of Nontypeable Haemophilus influenzae That Cause Otitis Media. Infect. Immun. 70: 3551-3556 [Abstract] [Full Text]  
• Sethi, S., Murphy, T. F. (2001). Bacterial Infection in Chronic Obstructive Pulmonary Disease in 2000: a State-of-the-Art Review. Clin. Microbiol. Rev. 14: 336-363 [Abstract] [Full Text]  
• Dawid, S., Grass, S., St. Geme, J. W. III (2001). Mapping of Binding Domains of Nontypeable Haemophilus influenzae HMW1 and HMW2 Adhesins. Infect. Immun. 69: 307-314 [Abstract] [Full Text]  
• Read, T. D., Satola, S. W., Farley, M. M. (2000). Nucleotide Sequence Analysis of Hypervariable Junctions of Haemophilus influenzae Pilus Gene Clusters. Infect. Immun. 68: 6896-6902 [Abstract] [Full Text]  
• St. Geme, J. W. III, Cutter, D. (2000). The Haemophilus influenzae Hia Adhesin Is an Autotransporter Protein That Remains Uncleaved at the C Terminus and Fully Cell Associated. J. Bacteriol. 182: 6005-6013 [Abstract] [Full Text]  
• Bolduc, G. R., Bouchet, V., Jiang, R.-Z., Geisselsoder, J., Truong-Bolduc, Q. C., Rice, P. A., Pelton, S. I., Goldstein, R. (2000). Variability of Outer Membrane Protein P1 and Its Evaluation as a Vaccine Candidate against Experimental Otitis Media due to Nontypeable Haemophilus influenzae: an Unambiguous, Multifaceted Approach. Infect. Immun. 68: 4505-4517 [Abstract] [Full Text]  
• van Schilfgaarde, M., van Ulsen, P., Eijk, P., Brand, M., Stam, M., Kouame, J., van Alphen, L., Dankert, J. (2000). Characterization of Adherence of Nontypeable Haemophilus influenzae to Human Epithelial Cells. Infect. Immun. 68: 4658-4665 [Abstract] [Full Text]  
• Frick, A. G., Joseph, T. D., Pang, L., Rabe, A. M., St. Geme, J. W. III, Look, D. C. (2000). Haemophilus influenzae Stimulates ICAM-1 Expression on Respiratory Epithelial Cells. J. Immunol. 164: 4185-4196 [Abstract] [Full Text]  
• Elkins, C., Yi, K., Olsen, B., Thomas, C., Thomas, K., Morse, S. (2000). Development of a Serological Test for Haemophilus ducreyi for Seroprevalence Studies. J. Clin. Microbiol. 38: 1520-1526 [Abstract] [Full Text]  
• Cope, L. D., Lafontaine, E. R., Slaughter, C. A., Hasemann, C. A. Jr., Aebi, C., Henderson, F. W., McCracken, G. H. Jr., Hansen, E. J. (1999). Characterization of the Moraxella catarrhalis uspA1 and uspA2 Genes and Their Encoded Products. J. Bacteriol. 181: 4026-4034 [Abstract] [Full Text]  
• Gousset, N., Rosenau, A., Sizaret, P.-Y., Quentin, R. (1999). Nucleotide Sequences of Genes Coding for Fimbrial Proteins in a Cryptic Genospecies of Haemophilus spp. Isolated from Neonatal and Genital Tract Infections. Infect. Immun. 67: 8-15 [Abstract] [Full Text]  
• Jiang, Z., Nagata, N., Molina, E., Bakaletz, L. O., Hawkins, H., Patel, J. A. (1999). Fimbria-Mediated Enhanced Attachment of Nontypeable Haemophilus influenzae to Respiratory Syncytial Virus-Infected Respiratory Epithelial Cells. Infect. Immun. 67: 187-192 [Abstract] [Full Text]  
• Krasan, G. P., Cutter, D., Block, S. L., St. Geme, J. W. III (1999). Adhesin Expression in Matched Nasopharyngeal and Middle Ear Isolates of Nontypeable Haemophilus influenzae from Children with Acute Otitis Media. Infect. Immun. 67: 449-454 [Abstract] [Full Text]  
• Mhlanga-Mutangadura, T., Morlin, G., Smith, A. L., Eisenstark, A., Golomb, M. (1998). Evolution of the Major Pilus Gene Cluster of Haemophilus influenzae. J. Bacteriol. 180: 4693-4703 [Abstract] [Full Text]