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Infection and Immunity, October 1998, p. 4624-4632, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Invasion by Salmonella typhimurium Induces Increased Expression of the LMP, MECL, and PA28 Proteasome Genes and Changes in the Peptide Repertoire of HLA-B27

Walter P. Maksymowych,^1,* Takashi Ikawa,² Akihiro Yamaguchi,² Makoto Ikeda,² Darrin McDonald,¹ Leila Laouar,¹ Riitta Lahesmaa,³ Naoto Tamura,² Arranny Khuong,¹ David T. Y. Yu,² and Kevin P. Kane¹

Departments of Medicine and Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada,¹ and Department of Medicine, University of California, Los Angeles,² and Roche Bioscience, Palo Alto,³ California

Received 10 November 1997/Returned for modification 26 January 1998/Accepted 23 June 1998

We have analyzed proteasomal adaptation and associated changes in the B27-bound peptide repertoire in response to cellular invasion with Salmonella. The peptide repertoire of HLA-B27 complexes was analyzed by two different methods: (i) high-pressure liquid chromatography (HPLC) profiles of newly synthesized peptides eluted from B27 following metabolic labeling with arginine and (ii) reactivities with two B27 monoclonal antibodies, Ye-2 and B27.M2, sensitive to peptide-induced conformational changes. LMP, MECL, and PA28 expression was analyzed by reverse transcription-PCR (RT-PCR) of mRNA and by Western blot analysis for LMP2. Invasion of HLA-B27-transfected HeLa cells by Salmonella typhimurium induced significant changes in the reactivities of HLA-B27 with these two antibodies, which was accompanied by significant quantitative and qualitative changes in the HPLC profile of peptides eluted from HLA-B27. We also observed increases in the RT-PCR values for the LMP2, LMP7, and MECL proteasome subunit genes, as well as the proteasomal activator PA28 and - genes, and increased expression of the LMP2 protein by Western blotting. Upregulation of LMP2, but not LMP7, gene expression showed a close correlation with the changes in antibody reactivities observed upon bacterial invasion. We observed similar changes in reactivity with the Ye-2 or the B27.M2 antibody of lymphoblastoid cells upon gamma interferon treatment, which significantly correlated with the increased RT-PCR values for the LMP2 gene. This was accompanied by consistent HPLC profile changes for eluted peptides. Thus, Salmonella invasion leads to serologically recognizable changes in the B27-bound peptide repertoire, which may include peptides of host origin potentially through modulation of proteasome LMP2 subunit expression and, as a consequence, proteasomal activities.

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^* Corresponding author. Mailing address: 562 Heritage Medical Research Center, University of Alberta, Edmonton, Alberta, Canada T6G 2S2. Phone: (403) 492-1964. Fax: (403) 492-6055. E-mail: maksymow{at}gpu.srv.ualberta.ca.

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Infection and Immunity, October 1998, p. 4624-4632, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

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