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Infection and Immunity, October 1998, p. 4726-4728, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Characterization of Outer Membrane Protein OmpU
of Vibrio cholerae O1
Noboru
Nakasone,* and
Masaaki
Iwanaga
Department of Bacteriology, Faculty of Medicine, University
of the Ryukyus, Nishihara, Okinawa 903-0215, Japan
Received 26 March 1998/Returned for modification 3 June
1998/Accepted 12 July 1998
The outer membrane protein OmpU of Vibrio cholerae O1
strain 86B3 was characterized with reference to colonization of the intestine by the organism. The purified OmpU exhibited a pI of 3.6. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it
migrated to 38, 32, and 110 kDa when the sample was heated at 100°C
for 2 min, 50°C for 15 min, and room temperature for 30 min,
respectively. The purified OmpU was not hemagglutinative. Anti-OmpU
serum did not agglutinate strain 86B3 or other V. cholerae organisms. OmpU adhered to the brush border of the
rabbit small intestine; adhesion of the organisms to the intestine
treated in advance with OmpU was not inhibited. Treating the organisms in advance with anti-OmpU Fab did not inhibit adhesion to the intestine. These results obtained in vitro suggest that OmpU is not
involved in the adhesion of V. cholerae to the
intestinal epithelium.
*
Corresponding author. Mailing address: Department of
Bacteriology, Faculty of Medicine, University of the Ryukyus,
Nishihara, Okinawa 903-0215, Japan. Phone: 81-98-895-3331, ext. 2287. Fax: 81-98-895-2951. E-mail:
noboru{at}med.u-ryukyu.ac.jp.
Infection and Immunity, October 1998, p. 4726-4728, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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