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Infection and Immunity, October 1998, p. 4742-4747, Vol. 66, No. 10
First Department of Internal
Medicine1 and
Department of Laboratory
Medicine,2 Nagoya University School of
Medicine, Nagoya, Japan, and
Division of Infectious Disease,
Department of Medicine, Vanderbilt University School of Medicine
and VA Medical Center, Nashville, Tennessee3
Received 6 April 1998/Returned for modification 15 June
1998/Accepted 21 July 1998
There is differential resolution of mucosal infiltration with
neutrophils and mononuclear cells following successful
Helicobacter pylori eradication. We investigated the
effects of H. pylori eradication on mucosal
interleukin-8 (IL-8) and IL-6 activity in relation to the
resolution of H. pylori-associated gastritis.
Eighty-one duodenal ulcer patients with H. pylori
infection received dual- or triple-treatment eradication therapy, and
mucosal biopsy specimens obtained at the initial and follow-up
endoscopic examinations were cultured in vitro for 24 h. The
levels of IL-8 and IL-6 were measured by enzyme-linked
immunosorbent assays. In the 42 patients in whom
H. pylori eradication failed, there was
little change in the numbers of neutrophils and mononuclear cells
infiltrating the mucosa and in IL-8 and IL-6 activity. In the
39 patients in whom H. pylori was eradicated,
there was normalization both in the numbers of
infiltrating neutrophils and in mucosal IL-8 activity, which
was evident within 1 month following therapy. In contrast, there was a gradual resolution of mononuclear cell infiltration over a 6-month period, accompanied by a gradual normalization in
IL-6 levels. Addition of H. pylori to
cultures of mucosal tissues induced a significant increase in
IL-8 activity in both uninfected control subjects and
patients from whom H. pylori was eradicated. However, this introduction yielded a significant increase in IL-6 activity only in the latter group. This study indicates a dichotomy in
the changes of mucosal IL-8 and IL-6 activity after
H. pylori eradication. The rapid normalization of
IL-8 after H. pylori eradication and the ability
of H. pylori cells to stimulate IL-8 in control tissues indicate that IL-8 induction is a part of the innate
(nonimmune) responses to this organism. In contrast, the
results of experiments analyzing IL-6 activity in cultured mucosal
tissues suggest that the gradual resolution of mucosal IL-6
activity and mononuclear infiltration after successful eradication
observed in vivo may reflect gradually diminishing residual immune
responses against H. pylori.
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Differential Normalization of Mucosal Interleukin-8 and
Interleukin-6 Activity after Helicobacter pylori
Eradication
*
Corresponding author. Mailing address: First Department
of Internal Medicine, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan. Phone: 81 52 744 2144. Fax:
81 52 744 2157. E-mail: kusugami{at}tsuru.med.nagoya-u.ac.jp.
Infection and Immunity, October 1998, p. 4742-4747, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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