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Infection and Immunity, October 1998, p. 4903-4909, Vol. 66, No. 10
Tuberculosis and Related Infections Unit,
Received 23 March 1998/Returned for modification 25 May
1998/Accepted 23 July 1998
We report here the mapping of T-cell-stimulatory determinants of
the GroES 10-kDa heat shock protein homologues from Mycobacterium leprae and Mycobacterium tuberculosis, which are
known as major immunogens in mycobacterial infections. Peripheral blood
mononuclear cells (PBMC) from treated tuberculoid leprosy or
lepromatous leprosy patients and from healthy household or hospital
staff contacts of the patients were cultured with 20 16-mer peptides
covering the entire sequences of both M. leprae and
M. tuberculosis GroES. The total number of recognized
peptides was found to be the largest in family contacts, while
responder frequencies to the individual tested peptides varied (5 to
80%) with specificity between the patient and contact groups.
Proliferative responses to some peptides showed positive or negative
associations of low statistical significance with DR and DQ alleles,
though responses to most GroES peptides were genetically permissive.
Notably, the sequence of the 25-40 peptide of M. leprae,
but not that of M. tuberculosis, was more frequently
stimulatory in tuberculoid leprosy patients than in either group of
sensitized healthy contacts. This peptide bound to a number of HLA-DR
molecules, of which HLA-DRB5*0101 had the strongest affinity. The
epitope core binding to this allele was localized to the 29-to-37
sequence, and its key residue was localized to the M. leprae-specific glutamic acid at position 32. This epitope may be
of interest for the development of a blood test- or skin test-based diagnostic reagent for tuberculoid leprosy, subject to
further clinical evaluation in untreated patients.
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
T-Cell Recognition of Mycobacterial GroES Peptides
in Thai Leprosy Patients and Contacts
*
Corresponding author. Present address: Department of
Oral Medicine and Pathology, UMDS at Guy's Hospital, Floor 28 Guy's
Tower, London SE1 9RT, United Kingdom. Phone: 171-955 4568. Fax:
171-955 4455. E-mail: j.ivanyi{at}umds.ac.uk.
Infection and Immunity, October 1998, p. 4903-4909, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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