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Infection and Immunity, October 1998, p. 4965-4970, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of Two Laminin-Binding Fimbriae, the Type 1 Fimbria of Salmonella enterica Serovar Typhimurium and the G Fimbria of Escherichia coli, as Plasminogen Receptors

Maini Kukkonen,1 Sirkku Saarela,1 Kaarina Lähteenmäki,1 Ulla Hynönen,1 Benita Westerlund-Wikström,1 Mikael Rhen,2 and Timo K. Korhonen1,*

Division of General Microbiology, Department of Biosciences, FIN-00014 University of Helsinki, Finland,1 and Microbiology and Tumor Biology Center, Karolinska Institute, 17177 Stockholm, Sweden2

Received 9 March 1998/Returned for modification 23 April 1998/Accepted 12 July 1998

Escherichia coli strains carrying recombinant plasmids encoding either the type 1 fimbria of Salmonella enterica serovar Typhimurium or the G fimbria of E. coli exhibited binding of human 125I-Glu-plasminogen and enhanced the tissue-type plasminogen activator-catalyzed conversion of plasminogen to plasmin. Purified type 1 or G fimbriae similarly bound plasminogen and enhanced its activation. The binding of plasminogen did not involve the characteristic carbohydrate-binding property of the fimbriae but was inhibited at low concentrations by the lysine analog varepsilon -aminocaproic acid. Because these fimbrial types bind to laminin of basement membranes (M. Kukkonen et al., Mol. Microbiol. 7:229-237, 1993; S. Saarela et al., Infect. Immun. 64:2857-2860, 1996), the results demonstrate a structural unity in the creation and targeting of bacterium-bound proteolytic plasmin activity to basement membranes.

* Corresponding author. Mailing address: Division of General Microbiology, Department of Biosciences, P.O. Box 56 (Viikinkaari 9), FIN-00014 University of Helsinki, Finland. Phone: 358-9-70859260. Fax: 358-9-70859262. E-mail: timo.korhonen{at}helsinki.fi.

Infection and Immunity, October 1998, p. 4965-4970, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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