This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roberts, W. C.
Right arrow Articles by Hanson, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roberts, W. C.
Right arrow Articles by Hanson, M. S.

 Previous Article  |  Next Article 

Infection and Immunity, November 1998, p. 5275-5285, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Analysis of Sequence Heterogeneity among Genes Encoding Decorin Binding Proteins A and B of Borrelia burgdorferi Sensu Lato

William C. Roberts, Brian A. Mullikin, Raju Lathigra, and Mark S. Hanson*

MedImmune, Inc., Gaithersburg, Maryland 20878

Received 22 April 1998/Returned for modification 10 July 1998/Accepted 28 August 1998

Immunization of mice with Borrelia burgdorferi decorin binding protein A (DbpA), one of two gene products of the dbpBA locus, has been shown recently to confer protection against challenge. Hyperimmune DbpA antiserum killed a large number of B. burgdorferi sensu lato isolates of diverse phylogeny and origin, suggesting conservation of the protective epitope(s). In order to evaluate the heterogeneity of DbpA and DbpB and to facilitate defining the conserved epitope(s) of these antigens, the sequences of the dbpA genes from 29 B. burgdorferi sensu lato isolates and of the dbpB genes from 15 B. burgdorferi sensu lato isolates were determined. The predicted DbpA sequences were fairly heterogeneous among the isolates (58.3 to 100% similarity), but DbpA sequences with the highest similarity tended to group into species previously defined by well-characterized chromosomal markers. In contrast, the predicted DbpB sequences were highly conserved (96.3 to 100% similarity). Substantial diversity in DbpA sequence was seen among isolates previously shown to be killed by antiserum against a single DbpA, suggesting that one or more conserved protective epitopes are composed of noncontiguous amino acids. The observation of individual dbpA alleles with sequence elements characteristic of more than one B. burgdorferi sensu lato species was consistent with a role for genetic recombination in the generation of dbpA diversity.

* Corresponding author. Mailing address: MedImmune, Inc., 35 West Watkins Mill Road, Gaithersburg, MD 20878. Phone: (301) 527-4264. Fax: (301) 527-4200. E-mail: hansonm{at}medimmune.com.

Infection and Immunity, November 1998, p. 5275-5285, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Barbour, A. G., Jasinskas, A., Kayala, M. A., Davies, D. H., Steere, A. C., Baldi, P., Felgner, P. L. (2008). A Genome-Wide Proteome Array Reveals a Limited Set of Immunogens in Natural Infections of Humans and White-Footed Mice with Borrelia burgdorferi. Infect. Immun. 76: 3374-3389 [Abstract] [Full Text]  
  • Goettner, G., Schulte-Spechtel, U., Hillermann, R., Liegl, G., Wilske, B., Fingerle, V. (2005). Improvement of Lyme Borreliosis Serodiagnosis by a Newly Developed Recombinant Immunoglobulin G (IgG) and IgM Line Immunoblot Assay and Addition of VlsE and DbpA Homologues. J. Clin. Microbiol. 43: 3602-3609 [Abstract] [Full Text]  
  • Pikas, D. S., Brown, E. L., Gurusiddappa, S., Lee, L. Y., Xu, Y., Hook, M. (2003). Decorin-binding Sites in the Adhesin DbpA from Borrelia burgdorferi: A SYNTHETIC PEPTIDE APPROACH. J. Biol. Chem. 278: 30920-30926 [Abstract] [Full Text]  
  • Miller, J. C., Stevenson, B. (2003). Immunological and genetic characterization of Borrelia burgdorferi BapA and EppA proteins. Microbiology 149: 1113-1125 [Abstract] [Full Text]  
  • Schulte-Spechtel, U., Lehnert, G., Liegl, G., Fingerle, V., Heimerl, C., Johnson, B. J. B., Wilske, B. (2003). Significant Improvement of the Recombinant Borrelia-Specific Immunoglobulin G Immunoblot Test by Addition of VlsE and a DbpA Homologue Derived from Borrelia garinii for Diagnosis of Early Neuroborreliosis. J. Clin. Microbiol. 41: 1299-1303 [Abstract] [Full Text]  
  • HEIKKILA, T., SEPPALA, I., SAXEN, H., PANELIUS, J., PELTOMAA, M., HUPPERTZ, H.-I., LAHDENNE, P. (2002). Cloning of the gene encoding the decorin-binding protein B (DbpB) in Borrelia burgdorferi sensu lato and characterisation of the antibody responses to DbpB in Lyme borreliosis. J Med Microbiol 51: 641-648 [Abstract] [Full Text]  
  • Liang, F. T., Nelson, F. K., Fikrig, E. (2002). DNA Microarray Assessment of Putative Borrelia burgdorferi Lipoprotein Genes. Infect. Immun. 70: 3300-3303 [Abstract] [Full Text]  
  • Heikkila, T., Seppala, I., Saxen, H., Panelius, J., Yrjanainen, H., Lahdenne, P. (2002). Species-Specific Serodiagnosis of Lyme Arthritis and Neuroborreliosis Due to Borrelia burgdorferi Sensu Stricto, B. afzelii, and B. garinii by Using Decorin Binding Protein A. J. Clin. Microbiol. 40: 453-460 [Abstract] [Full Text]  
  • Ulbrandt, N. D., Cassatt, D. R., Patel, N. K., Roberts, W. C., Bachy, C. M., Fazenbaker, C. A., Hanson, M. S. (2001). Conformational Nature of the Borrelia burgdorferi Decorin Binding Protein A Epitopes That Elicit Protective Antibodies. Infect. Immun. 69: 4799-4807 [Abstract] [Full Text]  
  • Carroll, J. A., Cordova, R. M., Garon, C. F. (2000). Identification of 11 pH-Regulated Genes in Borrelia burgdorferi Localizing to Linear Plasmids. Infect. Immun. 68: 6677-6684 [Abstract] [Full Text]  
  • Hanson, M. S., Patel, N. K., Cassatt, D. R., Ulbrandt, N. D. (2000). Evidence for Vaccine Synergy between Borrelia burgdorferi Decorin Binding Protein A and Outer Surface Protein A in the Mouse Model of Lyme Borreliosis. Infect. Immun. 68: 6457-6460 [Abstract] [Full Text]  
  • Haake, D. A. (2000). Spirochaetal lipoproteins and pathogenesis. Microbiology 146: 1491-1504 [Full Text]  
  • Miller, J. C., El-Hage, N., Babb, K., Stevenson, B. (2000). Borrelia burgdorferi B31 Erp Proteins That Are Dominant Immunoblot Antigens of Animals Infected with Isolate B31 Are Recognized by Only a Subset of Human Lyme Disease Patient Sera. J. Clin. Microbiol. 38: 1569-1574 [Abstract] [Full Text]  
  • Wang, G., van Dam, A. P., Schwartz, I., Dankert, J. (1999). Molecular Typing of Borrelia burgdorferi Sensu Lato: Taxonomic, Epidemiological, and Clinical Implications. Clin. Microbiol. Rev. 12: 633-653 [Abstract] [Full Text]  
  • Brown, E. L., Guo, B. P., O'Neal, P., Hook, M. (1999). Adherence of Borrelia burgdorferi. IDENTIFICATION OF CRITICAL LYSINE RESIDUES IN DbpA REQUIRED FOR DECORIN BINDING. J. Biol. Chem. 274: 26272-26278 [Abstract] [Full Text]  
  • Cassatt, D. R., Patel, N. K., Ulbrandt, N. D., Hanson, M. S. (1998). DbpA, but Not OspA, Is Expressed by Borrelia burgdorferi during Spirochetemia and Is a Target for Protective Antibodies. Infect. Immun. 66: 5379-5387 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»