This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lyashchenko, K. P.
Right arrow Articles by Gennaro, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lyashchenko, K. P.
Right arrow Articles by Gennaro, M. L.

 Previous Article  |  Next Article 

Infection and Immunity, November 1998, p. 5344-5349, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Diversity of Antigen Recognition by Serum Antibodies in Experimental Bovine Tuberculosis

Konstantin P. Lyashchenko,1,* John M. Pollock,2 Roberto Colangeli,1 and Maria Laura Gennaro1

Public Health Research Institute, New York, New York,1 and Veterinary Science Division, Department of Agriculture for Northern Ireland, Belfast, United Kingdom2

Received 15 June 1998/Returned for modification 17 July 1998/Accepted 14 August 1998

Tuberculosis in cattle remains a major zoonotic and economic problem in many countries. The standard diagnostic assay for bovine tuberculosis, the intradermal tuberculin test, has low accuracy. Therefore, alternative immunodiagnostic methods, such as serological assays, are needed for detection of infected animals. Development of an accurate serodiagnostic test requires a detailed understanding of the humoral immune responses during bovine tuberculosis and, in particular, identification of the key antigens of Mycobacterium bovis involved in antibody production. In this study, we characterized antibody responses in cattle experimentally infected with M. bovis. Sequential serum samples were collected every 3 to 4 weeks for up to 27 months postinfection. Circulating immunoglobulin G antibody levels were measured by an enzyme-linked immunosorbent assay using 12 highly purified recombinant proteins of M. bovis. Six proteins, ESAT-6, 14-kDa protein, MPT63, MPT70, MPT51, and MPT32, were identified as major seroreactive antigens in bovine tuberculosis. A remarkable animal-to-animal variation of antigen recognition by serum antibodies was observed. Kinetic analyses of the antibody production to individual antigens during infection revealed that the heterogeneous antigen recognition profile changed markedly in a given infected animal as disease progressed.

* Corresponding author. Mailing address: Public Health Research Institute, 455 First Ave., New York, NY 10016. Phone: (212) 576-8422. Fax: (212) 578-0804. E-mail: kostya{at}phri.nyu.edu.

Infection and Immunity, November 1998, p. 5344-5349, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Dean, G. S., Crawshaw, T. R., de la Rua-Domenech, R., Farrant, L., Greenwald, R., Higgins, R. J., Lyashchenko, K., Vordermeier, H. M., Twomey, D. F. (2009). Use of serological techniques for diagnosis of Mycobacterium bovis infection in a llama herd. Vet Rec. 165: 323-324 [Full Text]  
  • Green, L. R., Jones, C. C., Sherwood, A. L., Garkavi, I. V., Cangelosi, G. A., Thacker, T. C., Palmer, M. V., Waters, W. R., Rathe, C. V. (2009). Single-Antigen Serological Testing for Bovine Tuberculosis. CVI 16: 1309-1313 [Abstract] [Full Text]  
  • Greenwald, R., Lyashchenko, O., Esfandiari, J., Miller, M., Mikota, S., Olsen, J. H., Ball, R., Dumonceaux, G., Schmitt, D., Moller, T., Payeur, J. B., Harris, B., Sofranko, D., Waters, W. R., Lyashchenko, K. P. (2009). Highly Accurate Antibody Assays for Early and Rapid Detection of Tuberculosis in African and Asian Elephants. CVI 16: 605-612 [Abstract] [Full Text]  
  • Drewe, J. A., Dean, G. S., Michel, A. L., Pearce, G. P. (2009). Accuracy of three diagnostic tests for determining Mycobacterium bovis infection status in live-sampled wild meerkats (Suricata suricatta). jvdi 21: 31-39 [Abstract] [Full Text]  
  • Whelan, C., Shuralev, E., O'Keeffe, G., Hyland, P., Kwok, H. F., Snoddy, P., O'Brien, A., Connolly, M., Quinn, P., Groll, M., Watterson, T., Call, S., Kenny, K., Duignan, A., Hamilton, M. J., Buddle, B. M., Johnston, J. A., Davis, W. C., Olwill, S. A., Clarke, J. (2008). Multiplex Immunoassay for Serological Diagnosis of Mycobacterium bovis Infection in Cattle. CVI 15: 1834-1838 [Abstract] [Full Text]  
  • Harrington, N. P., Surujballi, O. P., Prescott, J. F., Duncan, J. R., Waters, W. R., Lyashchenko, K., Greenwald, R. (2008). Antibody Responses of Cervids (Cervus elaphus) following Experimental Mycobacterium bovis Infection and the Implications for Immunodiagnosis. CVI 15: 1650-1658 [Abstract] [Full Text]  
  • Cho, D., Collins, M. T. (2006). Comparison of the Proteosomes and Antigenicities of Secreted and Cellular Proteins Produced by Mycobacterium paratuberculosis. CVI 13: 1155-1161 [Abstract] [Full Text]  
  • Lyashchenko, K. P., Greenwald, R., Esfandiari, J., Olsen, J. H., Ball, R., Dumonceaux, G., Dunker, F., Buckley, C., Richard, M., Murray, S., Payeur, J. B., Andersen, P., Pollock, J. M., Mikota, S., Miller, M., Sofranko, D., Waters, W. R. (2006). Tuberculosis in Elephants: Antibody Responses to Defined Antigens of Mycobacterium tuberculosis, Potential for Early Diagnosis, and Monitoring of Treatment.. CVI 13: 722-732 [Abstract] [Full Text]  
  • Waters, W. R., Palmer, M. V., Bannantine, J. P., Greenwald, R., Esfandiari, J., Andersen, P., McNair, J., Pollock, J. M., Lyashchenko, K. P. (2005). Antibody Responses in Reindeer (Rangifer tarandus) Infected with Mycobacterium bovis. CVI 12: 727-735 [Abstract] [Full Text]  
  • Waters, W. R., Palmer, M. V., Bannantine, J. P., Whipple, D. L., Greenwald, R., Esfandiari, J., Andersen, P., McNair, J., Pollock, J. M., Lyashchenko, K. P. (2004). Antigen Recognition by Serum Antibodies in White-Tailed Deer (Odocoileus virginianus) Experimentally Infected with Mycobacterium bovis. CVI 11: 849-855 [Abstract] [Full Text]  
  • Lyashchenko, K., Whelan, A. O., Greenwald, R., Pollock, J. M., Andersen, P., Hewinson, R. G., Vordermeier, H. M. (2004). Association of Tuberculin-Boosted Antibody Responses with Pathology and Cell-Mediated Immunity in Cattle Vaccinated with Mycobacterium bovis BCG and Infected with M. bovis. Infect. Immun. 72: 2462-2467 [Abstract] [Full Text]  
  • Diez, S., Gomez, B. L., McEwen, J. G., Restrepo, A., Hay, R. J., Hamilton, A. J. (2003). Combined Use of Paracoccidioides brasiliensis Recombinant 27-Kilodalton and Purified 87-Kilodalton Antigens in an Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Paracoccidioidomycosis. J. Clin. Microbiol. 41: 1536-1542 [Abstract] [Full Text]  
  • Houghton, R. L., Lodes, M. J., Dillon, D. C., Reynolds, L. D., Day, C. H., McNeill, P. D., Hendrickson, R. C., Skeiky, Y. A. W., Sampaio, D. P., Badaro, R., Lyashchenko, K. P., Reed, S. G. (2002). Use of Multiepitope Polyproteins in Serodiagnosis of Active Tuberculosis. CVI 9: 883-891 [Abstract] [Full Text]  
  • Gooding, T. M., Johnson, P. D. R., Campbell, D. E., Hayman, J. A., Hartland, E. L., Kemp, A. S., Robins-Browne, R. M. (2001). Immune Response to Infection with Mycobacterium ulcerans. Infect. Immun. 69: 1704-1707 [Abstract] [Full Text]  
  • Rhodes, S. G., Gavier-Widen, D., Buddle, B. M., Whelan, A. O., Singh, M., Hewinson, R. G., Vordermeier, H. M. (2000). Antigen Specificity in Experimental Bovine Tuberculosis. Infect. Immun. 68: 2573-2578 [Abstract] [Full Text]  
  • Chen, L.-C., Goldman, D. L., Doering, T. L., Pirofski, L.-a., Casadevall, A. (1999). Antibody Response to Cryptococcus neoformans Proteins in Rodents and Humans. Infect. Immun. 67: 2218-2224 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»