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Infection and Immunity, November 1998, p. 5344-5349, Vol. 66, No. 11
Public Health Research Institute, New York,
New York,1 and
Veterinary Science
Division, Department of Agriculture for Northern Ireland, Belfast,
United Kingdom2
Received 15 June 1998/Returned for modification 17 July
1998/Accepted 14 August 1998
Tuberculosis in cattle remains a major zoonotic and economic
problem in many countries. The standard diagnostic assay for bovine
tuberculosis, the intradermal tuberculin test, has low accuracy.
Therefore, alternative immunodiagnostic methods, such as serological
assays, are needed for detection of infected animals. Development of an
accurate serodiagnostic test requires a detailed understanding of the
humoral immune responses during bovine tuberculosis and, in particular,
identification of the key antigens of Mycobacterium bovis
involved in antibody production. In this study, we characterized antibody responses in cattle experimentally infected with M. bovis. Sequential serum samples were collected every 3 to 4 weeks
for up to 27 months postinfection. Circulating immunoglobulin G
antibody levels were measured by an enzyme-linked immunosorbent assay
using 12 highly purified recombinant proteins of M. bovis. Six proteins, ESAT-6, 14-kDa protein, MPT63, MPT70, MPT51,
and MPT32, were identified as major seroreactive antigens in bovine
tuberculosis. A remarkable animal-to-animal variation of antigen
recognition by serum antibodies was observed. Kinetic analyses of
the antibody production to individual antigens during infection
revealed that the heterogeneous antigen recognition profile changed
markedly in a given infected animal as disease progressed.
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Diversity of Antigen Recognition by Serum
Antibodies in Experimental Bovine Tuberculosis
*
Corresponding author. Mailing address: Public Health
Research Institute, 455 First Ave., New York, NY 10016. Phone: (212) 576-8422. Fax: (212) 578-0804. E-mail:
kostya{at}phri.nyu.edu.
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