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Infection and Immunity, November 1998, p. 5393-5398, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Gene Discovery through Expressed Sequence Tag Sequencing in Trypanosoma cruzi

Ramiro E. Verdun,1 Nelson Di Paolo,1 Turan P. Urmenyi,2 Edson Rondinelli,2 Alberto C. C. Frasch,1 and Daniel O. Sanchez1,*

Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, Buenos Aires, Argentina,1 and Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil2

Received 15 May 1998/Returned for modification 2 July 1998/Accepted 10 August 1998

Analysis of expressed sequence tags (ESTs) constitutes a useful approach for gene identification that, in the case of human pathogens, might result in the identification of new targets for chemotherapy and vaccine development. As part of the Trypanosoma cruzi genome project, we have partially sequenced the 5' ends of 1,949 clones to generate ESTs. The clones were randomly selected from a normalized CL Brener epimastigote cDNA library. A total of 14.6% of the clones were homologous to previously identified T. cruzi genes, while 18.4% had significant matches to genes from other organisms in the database. A total of 67% of the ESTs had no matches in the database, and thus, some of them might be T. cruzi-specific genes. Functional groups of those sequences with matches in the database were constructed according to their putative biological functions. The two largest categories were protein synthesis (23.3%) and cell surface molecules (10.8%). The information reported in this paper should be useful for researchers in the field to analyze genes and proteins of their own interest.

* Corresponding author. Mailing address: Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, INTI (Ed. 24), Av. Gral Paz entre Constituyentes y Albarellos, 1650 San Martín, Provincia de Buenos Aires, Argentina. Phone: (54-1) 752-0021. Fax: (54-1) 752-9639. E-mail: dsanchez{at}inti.gov.ar.

Infection and Immunity, November 1998, p. 5393-5398, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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