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Infection and Immunity, November 1998, p. 5515-5519, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Transmission and Serial Propagation of
Enterocytozoon bieneusi from Humans and Rhesus Macaques in
Gnotobiotic Piglets
Ivanela
Kondova,1
Keith
Mansfield,2
Michael A.
Buckholt,1
Barry
Stein,1
Giovanni
Widmer,1
Angela
Carville,2
Andrew
Lackner,2 and
Saul
Tzipori1,*
Division of Infectious Disease, Tufts
University School of Veterinary Medicine, North Grafton, Massachusetts
01536,1 and
Division of Comparative
Pathology, New England Regional Primate Research Center,
Southborough, Massachusetts 017722
Received 22 April 1998/Returned for modification 22 June
1998/Accepted 15 July 1998
For over a decade Enterocytozoon bieneusi infections in
people with AIDS have been linked with chronic diarrhea and wasting. The slow scientific progress in treating these infections is attributed to the inability of investigators to cultivate the parasite, which has
also precluded evaluation of effective therapies. We report here
successful serial transmissions of E. bieneusi from
patients with AIDS and from macaques with AIDS to immunosuppressed
gnotobiotic piglets. One infected piglet was still excreting spores at
necropsy 50 days after an oral challenge. Spores in feces were detected microscopically by trichrome stain and by PCR and within enterocytes by
in situ hybridization and immunohistochemistry. E. bieneusi infection induced no symptoms. The development of an animal model for
E. bieneusi will open up new opportunities for
investigating this parasite.
*
Corresponding author. Mailing address: Division of
Infectious Disease, Tufts University School of Veterinary Medicine,
North Grafton, MA 01536. Phone: (508) 839-7955. Fax: (508) 839-7977. E-mail: stzipori{at}infonet.tufts.edu.
Infection and Immunity, November 1998, p. 5515-5519, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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