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Infection and Immunity, November 1998, p. 5515-5519, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Transmission and Serial Propagation of Enterocytozoon bieneusi from Humans and Rhesus Macaques in Gnotobiotic Piglets

Ivanela Kondova,1 Keith Mansfield,2 Michael A. Buckholt,1 Barry Stein,1 Giovanni Widmer,1 Angela Carville,2 Andrew Lackner,2 and Saul Tzipori1,*

Division of Infectious Disease, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536,1 and Division of Comparative Pathology, New England Regional Primate Research Center, Southborough, Massachusetts 017722

Received 22 April 1998/Returned for modification 22 June 1998/Accepted 15 July 1998

For over a decade Enterocytozoon bieneusi infections in people with AIDS have been linked with chronic diarrhea and wasting. The slow scientific progress in treating these infections is attributed to the inability of investigators to cultivate the parasite, which has also precluded evaluation of effective therapies. We report here successful serial transmissions of E. bieneusi from patients with AIDS and from macaques with AIDS to immunosuppressed gnotobiotic piglets. One infected piglet was still excreting spores at necropsy 50 days after an oral challenge. Spores in feces were detected microscopically by trichrome stain and by PCR and within enterocytes by in situ hybridization and immunohistochemistry. E. bieneusi infection induced no symptoms. The development of an animal model for E. bieneusi will open up new opportunities for investigating this parasite.

* Corresponding author. Mailing address: Division of Infectious Disease, Tufts University School of Veterinary Medicine, North Grafton, MA 01536. Phone: (508) 839-7955. Fax: (508) 839-7977. E-mail: stzipori{at}infonet.tufts.edu.

Infection and Immunity, November 1998, p. 5515-5519, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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