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Infection and Immunity, November 1998, p. 5555-5560, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Diversity in Protein Synthesis and Viability of Helicobacter pylori Coccoid Forms in Response to Various Stimuli

Hiromoto Mizoguchi,1,* Toshio Fujioka,1 Kenji Kishi,1 Akira Nishizono,2 Reiji Kodama,1 and Masaru Nasu1

Second Department of Internal Medicine1 and Department of Infectious Disease Control,2 Oita Medical University, Hasama-machi, Oita 879-5593, Japan

Received 29 April 1998/Returned for modification 17 June 1998/Accepted 13 August 1998

The viability of the coccoid forms of Helicobacter pylori was evaluated by assessing protein synthesis. Metabolic labeling studies showed the synthesis of proteins and the specific protein profiles of H. pylori coccoids produced under various conditions. Harsh conditions such as aerobiosis and starvation (lack of horse serum) in the culture did not affect the synthesis of proteins in the coccoids. Lowering of the pH to that of gastric secretions induced expression of several proteins in the coccoids. However, the coccoids produced under prolonged microaerobic conditions exhibited a profile of acid stress-induced protein expression different from that induced by aerobiosis or starvation. Our data suggest that coccoid H. pylori exhibits diversity in viability following exposure to different stresses and that the response to acid stress of coccoid H. pylori could be involved in infection of the host stomach.

* Corresponding author. Mailing address: Second Department of Internal Medicine, Oita Medical University, Hasama-machi, Oita 879-5593, Japan. Phone: 81-97-549-4411, ext. 5804. Fax: 81-97-549-4245.

Infection and Immunity, November 1998, p. 5555-5560, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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