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Infection and Immunity, November 1998, p. 5570-5575, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Protein (M9) Associated with Monoclonal Antibody-Mediated Agglutination of Mycoplasma gallisepticum Is a Member of the pMGA Family

Li Liu,1 D. Michael Payne,2 Vicky L. van Santen,1 Kevin Dybvig,3 and Victor S. Panangala1,*

Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 368491; Department of Anatomy and Cell Biology, University of North Texas Health Science Center, Fort Worth, Texas 761072; and Department of Comparative Medicine and Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 352943

Received 4 June 1998/Returned for modification 12 July 1998/Accepted 19 August 1998

A 62-kDa cell surface antigen (M9) of Mycoplasma gallisepticum PG31 that mediates antibody-induced agglutination of the organism was purified and subjected to N-terminal amino-acid sequencing. A 999-bp region of the cDNA encoding the M9 protein was generated by reverse transcription-PCR, and its nucleotide sequence was determined. PCR primers based on this sequence were used to screen a genomic DNA library of PG31. A full-length M9 protein-encoding gene was isolated and sequenced, revealing 96% nucleotide identity with the pMGA1.1 gene of M. gallisepticum S6. Sequence analyses of the M9 gene and flanking open reading frames that encode other pMGA family members suggest that a tandemly repeated GAA sequence may influence pMGA gene expression.

* Corresponding author. Mailing address: Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849. Phone: (334) 844-4953. Fax: (334) 844-4955. E-mail: pananvs{at}vetmed.auburn.edu.

Infection and Immunity, November 1998, p. 5570-5575, Vol. 66, No. 11
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.