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Infection and Immunity, December 1998, p. 5799-5804, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Secreted Effector Proteins of Salmonella dublin Act in Concert To Induce Enteritis

Michael A. Jones, Michael W. Wood, Paul B. Mullan,dagger Patricia R. Watson, Tim S. Wallis, and Edouard E. Galyov*

Institute for Animal Health, Compton, Berkshire RG20 7NN, United Kingdom

Received 15 July 1998/Returned for modification 19 August 1998/Accepted 10 September 1998

The ability of enteropathogenic salmonellae to recruit inflammatory cells and induce secretory responses in the infected ileum is considered to be a main feature in Salmonella-induced enteritis. Interactions between the pathogen and intestinal epithelial cells result in a variety of cellular responses mediating inflammation and fluid secretion. It is becoming apparent that proteins secreted by the Inv-Spa type III secretion system of Salmonella spp. play a key role in the induction of these responses. We have recently demonstrated that the SopB effector protein is translocated into eukaryotic cells via a Sip-dependent pathway and mediates inflammation and fluid secretion in infected ileal mucosa. However, SopB did not appear to be the only effector involved, as inactivation of the sopB gene only partially impaired enteropathogenicity. We suggested that at least some of such protein effectors are likely to be proteins of the same class as SopB, i.e., secreted effector proteins translocated into eukaroyotic cells via a Sip-dependent pathway. In this work, we identify SopD, another secreted protein belonging to the family of Sop effectors of Salmonella dublin. Using the cya reporter system we showed that SopD is translocated into eukaroyotic cells. We assessed the potential involvement of SopD in enteropathogenicity and found that inactivation of sopD has an additive effect in relation to the sopB mutation.

* Corresponding author. Mailing address: Institute for Animal Health, Compton, Berkshire RG20 7NN United Kingdom. Phone: (44) 1635 578411. Fax: (44) 1635 577263. E-mail: galyov{at}bbsrc.ac.uk.

dagger Present address: Department of Oncology, The Queen's University of Belfast, Belfast City Hospital, Belfast BT9 7AB Northern Ireland.

Infection and Immunity, December 1998, p. 5799-5804, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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