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Infection and Immunity, December 1998, p. 5921-5929, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Filamentous Hemagglutinin of Bordetella bronchiseptica Is Required for Efficient Establishment of Tracheal Colonization

Peggy A. Cotter,1 Ming H. Yuk,1 Seema Mattoo,1 Brian J. Akerley,1,dagger Jeff Boschwitz,2,3,Dagger David A. Relman,2,3 and Jeff F. Miller1,*

Department of Microbiology and Immunology, UCLA School of Medicine, University of California, Los Angeles, California 900951; Department of Microbiology and Immunology and Department of Medicine, Stanford University School of Medicine, Stanford, California 943052; and Veterans Affairs Palo Alto Health Care System, Palo Alto, California 943043

Received 24 June 1998/Returned for modification 11 August 1998/Accepted 1 September 1998

Adherence to ciliated respiratory epithelial cells is considered a critical early step in Bordetella pathogenesis. For Bordetella pertussis, the etiologic agent of whooping cough, several factors have been shown to mediate adherence to cells and cell lines in vitro. These putative adhesins include filamentous hemagglutinin (FHA), fimbriae, pertactin, and pertussis toxin. Determining the precise roles of each of these factors in vivo, however, has been difficult, due in part to the lack of natural-host animal models for use with B. pertussis. Using the closely related species Bordetella bronchiseptica, and by constructing both deletion mutation and ectopic expression mutants, we have shown that FHA is both necessary and sufficient for mediating adherence to a rat lung epithelial (L2) cell line. Using a rat model of respiratory infection, we have shown that FHA is absolutely required, but not sufficient, for tracheal colonization in healthy, unanesthetized animals. FHA was not required for initial tracheal colonization in anesthetized animals, however, suggesting that its role in establishment may be dedicated to overcoming the clearance action of the mucociliary escalator.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, UCLA School of Medicine, 10833 LeConte Ave., Los Angeles, CA 90095-1747. Phone: (310) 206-7926. Fax: (310) 206-3865. E-mail: jfmiller{at}ucla.edu.

dagger Present address: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.

Dagger Present address: Plan A, 759A Villa St., Mountain View, CA 94041.

Infection and Immunity, December 1998, p. 5921-5929, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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