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Infection and Immunity, December 1998, p. 6014-6016, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of the Helicobacter pylori VacA Toxin Domain Active in the Cell Cytosol

Marina de Bernard,1 Daniela Burroni,2 Emanuele Papini,1 Rino Rappuoli,2 John Telford,2 and Cesare Montecucco1,*

Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova1 and Centro Ricerche IRIS, CHIRON-Biocine Vaccines, 53100 Siena,2 Italy

Received 22 June 1998/Returned for modification 10 August 1998/Accepted 22 September 1998

Cells exposed to Helicobacter pylori toxin VacA develop large vacuoles which originate from massive swelling of membranous compartments at late stages of the endocytic pathway. When expressed in the cytosol, VacA induces vacuolization as it does when added from outside. This and other evidence indicate that VacA is a toxin capable of entering the cell cytosol, where it displays its activity. In this study, we have used cytosolic expression to identify the portion of the toxin molecule responsible for the vacuolating activity. VacA mutants with deletions at the C and N termini were generated, and their activity was analyzed upon expression in HeLa cells. We found that the vacuolating activity of VacA resides in the amino-terminal region, the whole of which is required for its intracellular activity.

* Corresponding author. Mailing address: Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, Via G. Colombo 3, 35121 Padva, Italy. Phone: 39-49-8276058. Fax: 39-49-8276049. E-mail: cesare{at}civ.bio.unipd.it.

Infection and Immunity, December 1998, p. 6014-6016, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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