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Infection and Immunity, December 1998, p. 6049-6053, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Enteropathogenic Escherichia coli Virulence Genes Encoding Secreted Signalling Proteins Are Essential for Modulation of Caco-2 Cell Electrolyte Transport

Georgina K. Collington,1,* Ian W. Booth,1 Michael S. Donnenberg,2 James B. Kaper,3 and Stuart Knutton1

Institute of Child Health, University of Birmingham, Birmingham B4 6NH, United Kingdom,1 and Division of Infectious Diseases2 and Centre for Vaccine Development,3 Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201

Received 8 June 1998/Returned for modification 24 July 1998/Accepted 29 September 1998

The pathophysiology of enteropathogenic Escherichia coli (EPEC) diarrhea remains uncertain. In vitro, EPEC stimulates a rapid increase in short-circuit current (Isc) across Caco-2 cell monolayers coincident with intimate attaching and effacing (A/E) bacterial adhesion. This study has examined the roles of specific EPEC virulence proteins in this Isc response. EPEC genes encoding EspA, EspB, and EspD, essential for signal transduction in host cells and A/E activity, were also required for modulation of Caco-2 electrolyte transport.

* Corresponding author. Mailing address: Institute of Child Health, University of Birmingham, Clinical Research Block, Whittall Street, Birmingham B4 6NH, United Kingdom. Phone: (44) 121-3338737. Fax: (44) 121-3338701. E-mail: g.k.collington{at}bham.ac.uk.

Infection and Immunity, December 1998, p. 6049-6053, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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