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Infection and Immunity, December 1998, p. 6058-6062, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Lactobacilli and Streptococci Induce Interleukin-12 (IL-12), IL-18, and Gamma Interferon Production in Human Peripheral Blood Mononuclear Cells

Minja Miettinen,1,2,* Sampsa Matikainen,1 Jaana Vuopio-Varkila,2 Jaana Pirhonen,1 Kari Varkila,3 Masashi Kurimoto,4 and Ilkka Julkunen1

Department of Virology1 and Department of Bacteriology,2 National Public Health Institute, 00300 Helsinki, and Orion Pharma, 02101 Espoo,3 Finland, and Fujisaki Institute, Hayashibara Biochemical Laboratories Inc., 675-1 Fujisaki, Okayama 702-8006, Japan4

Received 8 April 1998/Returned for modification 10 August 1998/Accepted 4 September 1998

Human peripheral blood mononuclear cells (PBMC) were stimulated with three nonpathogenic Lactobacillus strains and with one pathogenic Streptococcus pyogenes strain, and cytokine gene expression and protein production were analyzed. All bacteria strongly induced interleukin-1beta (IL-1beta ), IL-6, and tumor necrosis factor alpha mRNA expression and protein production. S. pyogenes was the most potent inducer of secretion of IL-12 and gamma interferon (IFN-gamma ), and two of three Lactobacillus strains induced IL-12 and IFN-gamma production. All strains induced IL-18 protein production. IL-10 and IL-4 production was induced weakly and not at all, respectively. Our data show that nonpathogenic lactobacilli and pathogenic streptococci can induce Th1 type cytokines IL-12, IL-18, and IFN-gamma in human PBMC.

* Corresponding author. Mailing address: Department of Virology, National Public Health Institute, Mannerheimintie 166, 00300 Helsinki, Finland. Phone: 358-9-47448303. Fax: 358-9-47448355. E-mail: minja.miettinen{at}ktl.fi.

Infection and Immunity, December 1998, p. 6058-6062, Vol. 66, No. 12
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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