This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Corthésy-Theulaz, I. E.
Right arrow Articles by Kraehenbuhl, J.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Corthésy-Theulaz, I. E.
Right arrow Articles by Kraehenbuhl, J.-P.

 Previous Article  |  Next Article 

Infect Immun, February 1998, p. 581-586, Vol. 66, No. 2
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Mice Are Protected from Helicobacter pylori Infection by Nasal Immunization with Attenuated Salmonella typhimurium phoPc Expressing Urease A and B Subunits

Irène E. Corthésy-Theulaz,1,* Sally Hopkins,2 Daniel Bachmann,1 Pierre F. Saldinger,1 Nadine Porta,1 Rainer Haas,3 Yan Zheng-Xin,4 Thomas Meyer,4 Hanifa Bouzourène,5 André L. Blum,1 and Jean-Pierre Kraehenbuhl2

Division of Gastroenterology, Department of Internal Medicine CHUV,1 and Institute of Pathology,5 Lausanne University, Lausanne, and Biochemistry Institute and ISREC, Epalinges,2 Switzerland, and Max von Pettenkofer Institut, Munich,3 and Max Planck Institut für Biologie, Tübingen,4 Germany

Received 27 August 1997/Returned for modification 22 October 1997/Accepted 28 November 1997

Live Salmonella typhimurium phoPc bacteria were tested as mucosal vaccine vectors to deliver Helicobacter pylori antigens. The genes encoding the A and B subunits of H. pylori urease were introduced into S. typhimurium phoPc and expressed under the control of a constitutive tac promoter (tac-ureAB) or a two-phase T7 expression system (cT7-ureAB). Both recombinant Salmonella strains expressed the two urease subunits in vitro and were used to nasally immunize BALB/c mice. The plasmid carrying cT7-ureAB was stably inherited by bacteria growing or persisting in the spleen, lungs, mesenteric or cervical lymph nodes, and Peyer's patches of immunized mice, while the plasmid carrying tac-ureAB was rapidly lost. Spleen and Peyer's patch CD4+ lymphocytes from mice immunized with S. typhimurium phoPc cT7-ureAB proliferated in vitro in response to urease, whereas cells from mice given S. typhimurium phoPc alone did not. Splenic CD4+ cells from mice immunized with phoPc cT7-ureAB secreted gamma interferon and interleukin 10, while Peyer's patch CD4+ cells did not secrete either cytokine. Specific H. pylori anti-urease immunoglobulin G1 (IgG1) and IgG2A antibodies were detected following immunization, confirming that both Th1- and Th2-type immune responses were generated by the live vaccine. Sixty percent of the mice (9 of 15) immunized with S. typhimurium phoPc cT7-ureAB were found to be resistant to infection by H. pylori, while all mice immunized with phoPc tac-ureAB (15 of 15) or phoPc (15 of 15) were infected. Our data demonstrate that H. pylori urease delivered nasally by using a vaccine strain of S. typhimurium can trigger Th1- and Th2-type responses and induce protective immunity against Helicobacter infection.

* Corresponding author. Mailing address: Department of Internal Medicine, Division of Gastroenterology, CHUV - BH-19N-624, CH-1011 Lausanne, Switzerland. Phone: 41 21 314 06 85. Fax: 41 21 314 06 84. E-mail: Irene.CorthesyTheulaz{at}ipharm.unil.ch.




This article has been cited by other articles:

  • Gao, L., Weck, M. N., Michel, A., Pawlita, M., Brenner, H. (2009). Association between Chronic Atrophic Gastritis and Serum Antibodies to 15 Helicobacter pylori Proteins Measured by Multiplex Serology. Cancer Res. 69: 2973-2980 [Abstract] [Full Text]  
  • Agarwal, K., Agarwal, S. (2008). Helicobacter pylori Vaccine: From Past to Future. Mayo Clin Proc. 83: 169-175 [Abstract] [Full Text]  
  • Graham, D Y, Opekun, A R, Osato, M S, El-Zimaity, H M T, Lee, C K, Yamaoka, Y, Qureshi, W A, Cadoz, M, Monath, T P (2004). Challenge model for Helicobacter pylori infection in human volunteers. Gut 53: 1235-1243 [Abstract] [Full Text]  
  • Rizos, K., Lattemann, C. T., Bumann, D., Meyer, T. F., Aebischer, T. (2003). Autodisplay: Efficacious Surface Exposure of Antigenic UreA Fragments from Helicobacter pylori in Salmonella Vaccine Strains. Infect. Immun. 71: 6320-6328 [Abstract] [Full Text]  
  • Di Genaro, M. S., Waidmann, M., Kramer, U., Hitziger, N., Bohn, E., Autenrieth, I. B. (2003). Attenuated Yersiniaenterocolitica Mutant Strains Exhibit Differential Virulence in Cytokine-Deficient Mice: Implications for the Development of Novel Live Carrier Vaccines. Infect. Immun. 71: 1804-1812 [Abstract] [Full Text]  
  • Corthesy-Theulaz, I., Corthesy, B., Bachmann, D., Velin, D., Kraehenbuhl, J.-P. (2003). Passive Immunity in Helicobacter-Challenged Neonatal Mice Conferred by Immunized Dams Lasts until Weaning. Infect. Immun. 71: 2226-2229 [Abstract] [Full Text]  
  • Panthel, K., Jechlinger, W., Matis, A., Rohde, M., Szostak, M., Lubitz, W., Haas, R. (2003). Generation of Helicobacter pylori Ghosts by PhiX Protein E-Mediated Inactivation and Their Evaluation as Vaccine Candidates. Infect. Immun. 71: 109-116 [Abstract] [Full Text]  
  • Banerjee, S, Medina-Fatimi, A, Nichols, R, Tendler, D, Michetti, M, Simon, J, Kelly, C P, Monath, T P, Michetti, P (2002). Safety and efficacy of low dose Escherichia coli enterotoxin adjuvant for urease based oral immunisation against Helicobacter pylori in healthy volunteers. Gut 51: 634-640 [Abstract] [Full Text]  
  • Londono-Arcila, P., Freeman, D., Kleanthous, H., O'Dowd, A. M., Lewis, S., Turner, A. K., Rees, E. L., Tibbitts, T. J., Greenwood, J., Monath, T. P., Darsley, M. J. (2002). Attenuated Salmonella enterica Serovar Typhi Expressing Urease Effectively Immunizes Mice against Helicobacter pylori Challenge as Part of a Heterologous Mucosal Priming-Parenteral Boosting Vaccination Regimen. Infect. Immun. 70: 5096-5106 [Abstract] [Full Text]  
  • Ohman, L, Franzen, L, Rudolph, U, Birnbaumer, L, Hornquist, E H. (2002). Regression of Peyer's patches in G{alpha}i2 deficient mice prior to colitis is associated with reduced expression of Bcl-2 and increased apoptosis. Gut 51: 392-397 [Abstract] [Full Text]  
  • Raghavan, S., Svennerholm, A.-M., Holmgren, J. (2002). Effects of Oral Vaccination and Immunomodulation by Cholera Toxin on Experimental Helicobacter pylori Infection, Reinfection, and Gastritis. Infect. Immun. 70: 4621-4627 [Abstract] [Full Text]  
  • Kang, H. Y., Srinivasan, J., Curtiss, R. III (2002). Immune Responses to Recombinant Pneumococcal PspA Antigen Delivered by Live Attenuated Salmonella enterica Serovar Typhimurium Vaccine. Infect. Immun. 70: 1739-1749 [Abstract] [Full Text]  
  • Bumann, D. (2001). Regulated Antigen Expression in Live Recombinant Salmonella enterica Serovar Typhimurium Strongly Affects Colonization Capabilities and Specific CD4+-T-Cell Responses. Infect. Immun. 69: 7493-7500 [Abstract] [Full Text]  
  • Bumann, D. (2001). In Vivo Visualization of Bacterial Colonization, Antigen Expression, and Specific T-Cell Induction following Oral Administration of Live Recombinant Salmonella enterica Serovar Typhimurium. Infect. Immun. 69: 4618-4626 [Abstract] [Full Text]  
  • Bonenfant, C., Dimier-Poisson, I., Velge-Roussel, F., Buzoni-Gatel, D., Del Giudice, G., Rappuoli, R., Bout, D. (2001). Intranasal Immunization with SAG1 and Nontoxic Mutant Heat-Labile Enterotoxins Protects Mice against Toxoplasma gondii. Infect. Immun. 69: 1605-1612 [Abstract] [Full Text]  
  • Lucas, B., Bumann, D., Walduck, A., Koesling, J., Develioglu, L., Meyer, T. F., Aebischer, T. (2001). Adoptive Transfer of CD4+ T Cells Specific for Subunit A of Helicobacter pylori Urease Reduces H. pylori Stomach Colonization in Mice in the Absence of Interleukin-4 (IL-4)/IL-13 Receptor Signaling. Infect. Immun. 69: 1714-1721 [Abstract] [Full Text]  
  • Solnick, J. V., Schauer, D. B. (2001). Emergence of Diverse Helicobacter Species in the Pathogenesis of Gastric and Enterohepatic Diseases. Clin. Microbiol. Rev. 14: 59-97 [Abstract] [Full Text]  
  • Aebischer, T., Laforsch, S., Hurwitz, R., Brombacher, F., Meyer, T. F. (2001). Immunity against Helicobacter pylori: Significance of Interleukin-4 Receptor {alpha} Chain Status and Gender of Infected Mice. Infect. Immun. 69: 556-558 [Abstract] [Full Text]  
  • Keenan, J., Oliaro, J., Domigan, N., Potter, H., Aitken, G., Allardyce, R., Roake, J. (2000). Immune Response to an 18-Kilodalton Outer Membrane Antigen Identifies Lipoprotein 20 as a Helicobacter pylori Vaccine Candidate. Infect. Immun. 68: 3337-3343 [Abstract] [Full Text]  
  • RUIZ-BUSTOS, E., SIERRA-BELTRAN, A., ROMERO, M.J., RODRIGUEZ-JARAMILLO, C., ASCENCIO, F. (2000). Protection of BALB/c mice against experimental Helicobacter pylori infection by oral immunisation with H. pylori heparan sulphate-binding proteins coupled to cholera toxin {beta}-subunit. J Med Microbiol 49: 535-541 [Abstract] [Full Text]  
  • Solnick, J. V., Canfield, D. R., Hansen, L. M., Torabian, S. Z. (2000). Immunization with Recombinant Helicobacter pylori Urease in Specific-Pathogen-Free Rhesus Monkeys (Macaca mulatta). Infect. Immun. 68: 2560-2565 [Abstract] [Full Text]  
  • Angelakopoulos, H., Hohmann, E. L. (2000). Pilot Study of phoP/phoQ-Deleted Salmonella enterica Serovar Typhimurium Expressing Helicobacter pylori Urease in Adult Volunteers. Infect. Immun. 68: 2135-2141 [Abstract] [Full Text]  
  • Velge-Roussel, F., Marcelo, P., Lepage, A. C., Buzoni-Gatel, D., Bout, D. T. (2000). Intranasal Immunization with Toxoplasma gondii SAG1 Induces Protective Cells into Both NALT and GALT Compartments. Infect. Immun. 68: 969-972 [Abstract] [Full Text]  
  • Ward, S. J., Douce, G., Figueiredo, D., Dougan, G., Wren, B. W. (1999). Immunogenicity of a Salmonella typhimurium aroA aroD Vaccine Expressing a Nontoxic Domain of Clostridium difficile Toxin A. Infect. Immun. 67: 2145-2152 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»