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Infect Immun, February 1998, p. 878-881, Vol. 66, No. 2
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Expression of beta -Defensin Genes in Bovine Alveolar Macrophages

Lisa K. Ryan,1 Janice Rhodes,2 Meenakshi Bhat,3 and Gill Diamond2,*

Immunotoxicology Branch, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 277111; Department of Anatomy, Cell Biology and Injury Sciences, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark, New Jersey 071032; and School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina 275143

Received 29 July 1997/Returned for modification 21 October 1997/Accepted 14 November 1997

Bovine alveolar macrophages (BAM) were examined for the expression of beta -defensins and to determine whether their expression could be upregulated by bacterial lipopolysaccharide (LPS), as observed with beta -defensins expressed in bovine tracheal epithelial cells. Four beta -defensins were expressed constitutively in BAM, with bovine neutrophil beta -defensin (BNBD)-4 and BNBD-5 being the most predominant. This is the first evidence of beta -defensin gene expression in a mature myeloid cell. LPS had no effect on beta -defensin expression in BAM, even though tumor necrosis factor alpha (TNF-alpha ) production was induced. Nonbacterial inflammatory particles had little effect on beta -defensin gene expression or TNF-alpha production in BAM. We hypothesize that constitutively expressed beta -defensins of alveolar macrophages may have a role in lung host defense.

* Corresponding author. Mailing address: Department of Anatomy, Cell Biology and Injury Sciences, UMDNJ-New Jersey Medical School, 185 South Orange Ave., Newark, NJ 07103. Phone: (973) 972-3324. Fax: (973) 972-7489. E-mail: gdiamond{at}umdnj.edu.




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