This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tang, P.
Right arrow Articles by Finlay, B. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tang, P.
Right arrow Articles by Finlay, B. B.

 Previous Article  |  Next Article 

Infect Immun, March 1998, p. 1106-1112, Vol. 66, No. 3
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Listeria monocytogenes Invasion of Epithelial Cells Requires the MEK-1/ERK-2 Mitogen-Activated Protein Kinase Pathway

Patrick Tang,1,2 Claire L. Sutherland,2 Michael R. Gold,2 and B. Brett Finlay1,2,3,*

The Biotechnology Laboratory1 and the Departments of Microbiology and Immunology2 and Biochemistry and Molecular Biology,3 University of British Columbia, Vancouver, British Columbia, Canada

Received 24 September 1997/Returned for modification 27 October 1997/Accepted 16 December 1997

PD98059, a specific inhibitor of MEK-1 mitogen-activated protein (MAP) kinase kinase, blocked Listeria monocytogenes invasion into HeLa epithelial cells. The effects of PD98059 were reversible, as adherent extracellular bacteria were internalized upon removal of the drug. Previously, we reported that L. monocytogenes could activate ERK-1 and ERK-2 MAP kinases through the action of listeriolysin O (LLO) on the host cell (P. Tang, I. Rosenshine, P. Cossart, and B. B. Finlay, Infect. Immun. 64:2359-2361, 1996). We have now found that two other MAP kinase pathways, those of p38 MAP kinase and c-Jun N-terminal kinase, are also activated by wild-type L. monocytogenes. Mutants lacking functional LLO (hly mutants) were still invasive but only activated ERK-2 and only activated it at later (90-min) postinfection times. Two inhibitors of L. monocytogenes invasion, cytochalasin D, which disrupts actin polymerization, and wortmannin, which blocks phosphatidylinositol (PI) 3-kinase activity, did not block ERK-2 activation by wild-type L. monocytogenes and hly mutants. However, genistein, an inhibitor of tyrosine kinases, and PD98059 both blocked invasion and decreased ERK-2 activation. These results suggest that MEK-1 and ERK-2 activities are essential for L. monocytogenes invasion into host epithelial cells. This is the first report to show that a MAP kinase pathway is required for bacterial invasion.

* Corresponding author. Mailing address: Biotechnology Laboratory, #237-6174 University Blvd., University of British Columbia, Vancouver, B.C., Canada V6T 1Z3. Phone: (604) 822-2210. Fax: (604) 822-9830. E-mail: bfinlay{at}unixg.ubc.ca.




This article has been cited by other articles:

  • Noor, S., Goldfine, H., Tucker, D. E., Suram, S., Lenz, L. L., Akira, S., Uematsu, S., Girotti, M., Bonventre, J. V., Breuel, K., Williams, D. L., Leslie, C. C. (2008). Activation of Cytosolic Phospholipase A2{alpha} in Resident Peritoneal Macrophages by Listeria monocytogenes Involves Listeriolysin O and TLR2. J. Biol. Chem. 283: 4744-4755 [Abstract] [Full Text]  
  • MacCallum, A., Haddock, G., Everest, P. H. (2005). Campylobacter jejuni activates mitogen-activated protein kinases in Caco-2 cell monolayers and in vitro infected primary human colonic tissue. Microbiology 151: 2765-2772 [Abstract] [Full Text]  
  • Jimenez de Bagues, M. P., Gross, A., Terraza, A., Dornand, J. (2005). Regulation of the Mitogen-Activated Protein Kinases by Brucella spp. Expressing a Smooth and Rough Phenotype: Relationship to Pathogen Invasiveness. Infect. Immun. 73: 3178-3183 [Abstract] [Full Text]  
  • Samaj, J., Baluska, F., Voigt, B., Schlicht, M., Volkmann, D., Menzel, D. (2004). Endocytosis, Actin Cytoskeleton, and Signaling. Plant Physiol. 135: 1150-1161 [Full Text]  
  • Okahashi, N., Inaba, H., Nakagawa, I., Yamamura, T., Kuboniwa, M., Nakayama, K., Hamada, S., Amano, A. (2004). Porphyromonas gingivalis Induces Receptor Activator of NF-{kappa}B Ligand Expression in Osteoblasts through the Activator Protein 1 Pathway. Infect. Immun. 72: 1706-1714 [Abstract] [Full Text]  
  • Dramsi, S., Cossart, P. (2003). Listeriolysin O-Mediated Calcium Influx Potentiates Entry of Listeria monocytogenes into the Human Hep-2 Epithelial Cell Line. Infect. Immun. 71: 3614-3618 [Abstract] [Full Text]  
  • Zhou, X., Giron, J. A., Torres, A. G., Crawford, J. A., Negrete, E., Vogel, S. N., Kaper, J. B. (2003). Flagellin of Enteropathogenic Escherichia coli Stimulates Interleukin-8 Production in T84 Cells. Infect. Immun. 71: 2120-2129 [Abstract] [Full Text]  
  • Betis, F., Brest, P., Hofman, V., Guignot, J., Bernet-Camard, M.-F., Rossi, B., Servin, A., Hofman, P. (2003). The Afa/Dr Adhesins of Diffusely Adhering Escherichia coli Stimulate Interleukin-8 Secretion, Activate Mitogen-Activated Protein Kinases, and Promote Polymorphonuclear Transepithelial Migration in T84 Polarized Epithelial Cells. Infect. Immun. 71: 1068-1074 [Abstract] [Full Text]  
  • Copp, J., Marino, M., Banerjee, M., Ghosh, P., van der Geer, P. (2003). Multiple Regions of Internalin B Contribute to Its Ability to Turn on the Ras-Mitogen-activated Protein Kinase Pathway. J. Biol. Chem. 278: 7783-7789 [Abstract] [Full Text]  
  • Blumenthal, A., Ehlers, S., Ernst, M., Flad, H.-D., Reiling, N. (2002). Control of Mycobacterial Replication in Human Macrophages: Roles of Extracellular Signal-Regulated Kinases 1 and 2 and p38 Mitogen-Activated Protein Kinase Pathways. Infect. Immun. 70: 4961-4967 [Abstract] [Full Text]  
  • Dahan, S., Busuttil, V., Imbert, V., Peyron, J.-F., Rampal, P., Czerucka, D. (2002). Enterohemorrhagic Escherichia coli Infection Induces Interleukin-8 Production via Activation of Mitogen-Activated Protein Kinases and the Transcription Factors NF-{kappa}B and AP-1 in T84 Cells. Infect. Immun. 70: 2304-2310 [Abstract] [Full Text]  
  • Lehner, M. D., Schwoebel, F., Kotlyarov, A., Leist, M., Gaestel, M., Hartung, T. (2002). Mitogen-Activated Protein Kinase-Activated Protein Kinase 2-Deficient Mice Show Increased Susceptibility to Listeria monocytogenes Infection. J. Immunol. 168: 4667-4673 [Abstract] [Full Text]  
  • Kohler, H., Rodrigues, S. P., McCormick, B. A. (2002). Shigella flexneri Interactions with the Basolateral Membrane Domain of Polarized Model Intestinal Epithelium: Role of Lipopolysaccharide in Cell Invasion and in Activation of the Mitogen-Activated Protein Kinase ERK. Infect. Immun. 70: 1150-1158 [Abstract] [Full Text]  
  • Tse, H. M., Josephy, S. I., Chan, E. D., Fouts, D., Cooper, A. M. (2002). Activation of the Mitogen-Activated Protein Kinase Signaling Pathway Is Instrumental in Determining the Ability of Mycobacterium avium to Grow in Murine Macrophages. J. Immunol. 168: 825-833 [Abstract] [Full Text]  
  • Mynott, T. L., Crossett, B., Prathalingam, S. R. (2002). Proteolytic Inhibition of Salmonella enterica Serovar Typhimurium-Induced Activation of the Mitogen-Activated Protein Kinases ERK and JNK in Cultured Human Intestinal Cells. Infect. Immun. 70: 86-95 [Abstract] [Full Text]  
  • Watanabe, K., Yilmaz, O., Nakhjiri, S. F., Belton, C. M., Lamont, R. J. (2001). Association of Mitogen-Activated Protein Kinase Pathways with Gingival Epithelial Cell Responses to Porphyromonas gingivalis Infection. Infect. Immun. 69: 6731-6737 [Abstract] [Full Text]  
  • de Grado, M., Rosenberger, C. M., Gauthier, A., Vallance, B. A., Finlay, B. B. (2001). Enteropathogenic Escherichia coli Infection Induces Expression of the Early Growth Response Factor by Activating Mitogen-Activated Protein Kinase Cascades in Epithelial Cells. Infect. Immun. 69: 6217-6224 [Abstract] [Full Text]  
  • Ellington, J. K., Elhofy, A., Bost, K. L., Hudson, M. C. (2001). Involvement of Mitogen-Activated Protein Kinase Pathways in Staphylococcus aureus Invasion of Normal Osteoblasts. Infect. Immun. 69: 5235-5242 [Abstract] [Full Text]  
  • Vazquez-Boland, J. A., Kuhn, M., Berche, P., Chakraborty, T., Dominguez-Bernal, G., Goebel, W., Gonzalez-Zorn, B., Wehland, J., Kreft, J. (2001). Listeria Pathogenesis and Molecular Virulence Determinants. Clin. Microbiol. Rev. 14: 584-640 [Abstract] [Full Text]  
  • Ibata-Ombetta, S., Jouault, T., Trinel, P.-A., Poulain, D. (2001). Role of extracellular signal-regulated protein kinase cascade in macrophage killing of Candida albicans. J. Leukoc. Biol. 70: 149-154 [Abstract] [Full Text]  
  • Czerucka, D., Dahan, S., Mograbi, B., Rossi, B., Rampal, P. (2001). Implication of Mitogen-Activated Protein Kinases in T84 Cell Responses to Enteropathogenic Escherichia coli Infection. Infect. Immun. 69: 1298-1305 [Abstract] [Full Text]  
  • Czerucka, D., Dahan, S., Mograbi, B., Rossi, B., Rampal, P. (2000). Saccharomyces boulardii Preserves the Barrier Function and Modulates the Signal Transduction Pathway Induced in Enteropathogenic Escherichia coli-Infected T84 Cells. Infect. Immun. 68: 5998-6004 [Abstract] [Full Text]  
  • Sahni, S. K., Turpin, L. C., Brown, T. L., Sporn, L. A. (1999). Involvement of Protein Kinase C in Rickettsia rickettsii-Induced Transcriptional Activation of the Host Endothelial Cell. Infect. Immun. 67: 6418-6423 [Abstract] [Full Text]  
  • Procyk, K. J., Rippo, M. R., Testi, R., Hoffmann, F., Parker, P. J., Baccarini, M. (1999). Distinct Mechanisms Target Stress and Extracellular Signal-Activated Kinase 1 and Jun N-Terminal Kinase During Infection of Macrophages with Salmonella. J. Immunol. 163: 4924-4930 [Abstract] [Full Text]  
  • Procyk, K. J., Kovarik, P., von Gabain, A., Baccarini, M. (1999). Salmonella typhimurium and Lipopolysaccharide Stimulate Extracellularly Regulated Kinase Activation in Macrophages by a Mechanism Involving Phosphatidylinositol 3-Kinase and Phospholipase D as Novel Intermediates. Infect. Immun. 67: 1011-1017 [Abstract] [Full Text]  
  • Wells, C. L., Jechorek, R. P., Kinneberg, K. M., Debol, S. M., Erlandsen, S. L. (1999). The Isoflavone Genistein Inhibits Internalization of Enteric Bacteria by Cultured Caco-2 and HT-29 Enterocytes. J. Nutr. 129: 634-640 [Abstract] [Full Text]  
  • Dumenil, G., Olivo, J.C., Pellegrini, S., Fellous, M., Sansonetti, P.J., Nhieu, G. T. V. (1998). Interferon alpha  Inhibits a Src-mediated Pathway Necessary for Shigella-induced Cytoskeletal Rearrangements in Epithelial Cells. JCB 143: 1003-1012 [Abstract] [Full Text]  
  • Guzman-Verri, C., Chaves-Olarte, E., von Eichel-Streiber, C., Lopez-Goni, I., Thelestam, M., Arvidson, S., Gorvel, J.-P., Moreno, E. (2001). GTPases of the Rho Subfamily Are Required for Brucella abortus Internalization in Nonprofessional Phagocytes. DIRECT ACTIVATION OF Cdc42. J. Biol. Chem. 276: 44435-44443 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»