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Infect Immun, March 1998, p. 1181-1189, Vol. 66, No. 3
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Human Platelets Damage Aspergillus fumigatus Hyphae and May Supplement Killing by Neutrophils

Laurent Christin,1 Deborah R. Wysong,1 Tova Meshulam,1 Ryan Hastey,2 Elizabeth R. Simons,2 and Richard D. Diamond1,*

Department of Medicine, Section of Infectious Diseases, Boston Medical Center,1 and Department of Biochemistry, Boston University School of Medicine,2 Boston, Massachusetts

Received 29 April 1997/Returned for modification 20 June 1997/Accepted 4 December 1997

Neutropenia is considered a significant risk factor for invasive aspergillosis but is almost always associated with concurrent thrombocytopenia. Studies determined that platelets, like neutrophils, attached to cell walls of the invasive hyphal form of Aspergillus fumigatus. Organisms were damaged as shown by loss of cell wall integrity in scanning laser confocal microscopy and release of defined hyphal surface glycoproteins. Rapid expression appearance of surface antigen CD63 and release of markers of platelet degranulation confirmed activation during attachment to hyphae. Optimal platelet activation required opsonization of hyphae with fresh or heat-inactivated whole plasma. These effects of opsonization with whole plasma could not be duplicated by pooled human serum, immunoglobulin G, or fibrinogen, whether used separately or combined. Thus, platelets in the presence of whole plasma have the potential to play an important role in normal host defenses against invasive aspergillosis.

* Corresponding author. Mailing address: Boston Medical Center, Room E-336, Section of Infectious Diseases, 88 E. Newton St., Boston, MA 02118. Phone: (617) 638-7909. Fax: (617) 638-8070. E-mail: rdiamond{at}med-med1.bu.edu.




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