This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schulte, R.
Right arrow Articles by Autenrieth, I. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schulte, R.
Right arrow Articles by Autenrieth, I. B.

 Previous Article  |  Next Article 

Infect Immun, March 1998, p. 1216-1224, Vol. 66, No. 3
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Yersinia enterocolitica-Induced Interleukin-8 Secretion by Human Intestinal Epithelial Cells Depends on Cell Differentiation

Ralf Schulte* and Ingo B. Autenrieth

Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität München, D-80336 Munich, Germany

Received 25 August 1997/Returned for modification 3 October 1997/Accepted 3 December 1997

In response to bacterial entry epithelial cells up-regulate expression and secretion of various proinflammatory cytokines, including interleukin-8 (IL-8). We studied Yersinia enterocolitica O:8-induced IL-8 secretion by intestinal epithelial cells as a function of cell differentiation. For this purpose, human T84 intestinal epithelial cells were grown on permeable supports, which led to the formation of tight monolayers of polarized intestinal epithelial cells. To analyze IL-8 secretion as a function of cell differentiation, T84 monolayers were infected from the apical or basolateral side at different stages of differentiation. Both virulent (plasmid-carrying) and nonvirulent (plasmid-cured) Y. enterocolitica strains invaded nondifferentiated T84 cells from the apical side. Yersinia invasion into T84 cells was followed by secretion of IL-8. After polarized differentiation of T84 cells Y. enterocolitica was no longer able to invade from the apical side or to induce IL-8 secretion by T84 cells. However, Y. enterocolitica invaded and induced IL-8 secretion by polarized T84 cells after infection from the basolateral side. Basolateral invasion required the presence of the Yersinia invasion locus, inv, suggesting beta 1 integrin-mediated cell invasion. After basolateral infection, Yersinia-induced IL-8 secretion was not strictly dependent on cell invasion. Thus, although the plasmid-carrying Y. enterocolitica strain did not significantly invade T84 cells, it induced significant IL-8 secretion. Taken together, these data show that Yersinia-triggered IL-8 secretion by intestinal epithelial cells depends on cell differentiation and might be induced by invasion as well as by basolateral adhesion, suggesting that invasion is not essential for triggering IL-8 production. Whether IL-8 secretion is involved in the pathogenesis of Yersinia-induced abscess formation in Peyer's patch tissue remains to be shown.

* Corresponding author. Mailing address: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität München, Pettenkoferstrasse 9a, D-80336 Munich, Germany. Phone: 49-89-51 60 52 59. Fax: 49-89-53 80 584. E-mail: schulte{at}m3401.mpk.med.uni-muenchen.de.




This article has been cited by other articles:

  • Odenbreit, S., Swoboda, K., Barwig, I., Ruhl, S., Boren, T., Koletzko, S., Haas, R. (2009). Outer Membrane Protein Expression Profile in Helicobacter pylori Clinical Isolates. Infect. Immun. 77: 3782-3790 [Abstract] [Full Text]  
  • Zheng, J., Meng, J., Zhao, S., Singh, R., Song, W. (2008). Campylobacter-Induced Interleukin-8 Secretion in Polarized Human Intestinal Epithelial Cells Requires Campylobacter-Secreted Cytolethal Distending Toxin- and Toll-Like Receptor-Mediated Activation of NF-{kappa}B. Infect. Immun. 76: 4498-4508 [Abstract] [Full Text]  
  • Frick, J. S., Fink, K., Kahl, F., Niemiec, M. J., Quitadamo, M., Schenk, K., Autenrieth, I. B. (2007). Identification of Commensal Bacterial Strains That Modulate Yersinia enterocolitica and Dextran Sodium Sulfate-Induced Inflammatory Responses: Implications for the Development of Probiotics. Infect. Immun. 75: 3490-3497 [Abstract] [Full Text]  
  • McNally, A., Dalton, T., Ragione, R. M. L., Stapleton, K., Manning, G., Newell, D. G. (2006). Yersinia enterocolitica isolates of differing biotypes from humans and animals are adherent, invasive and persist in macrophages, but differ in cytokine secretion profiles in vitro.. J Med Microbiol 55: 1725-1734 [Abstract] [Full Text]  
  • Buhler, O. T., Wiedig, C. A., Schmid, Y., Grassl, G. A., Bohn, E., Autenrieth, I. B. (2006). The Yersinia enterocolitica Invasin Protein Promotes Major Histocompatibility Complex Class I- and Class II-Restricted T-Cell Responses. Infect. Immun. 74: 4322-4329 [Abstract] [Full Text]  
  • Schmid, Y., Grassl, G. A., Buhler, O. T., Skurnik, M., Autenrieth, I. B., Bohn, E. (2004). Yersinia enterocolitica Adhesin A Induces Production of Interleukin-8 in Epithelial Cells. Infect. Immun. 72: 6780-6789 [Abstract] [Full Text]  
  • Krauss-Etschmann, S., Sammler, E., Koletzko, S., Konstantopoulos, N., Aust, D., Gebert, B., Luckow, B., Reinhardt, D., Schendel, D. J. (2003). Chemokine Receptor 5 Expression in Gastric Mucosa of Helicobacter pylori-Infected and Noninfected Children. CVI 10: 22-29 [Abstract] [Full Text]  
  • SCHULTE, R., GRASSL, G. A., PREGER, S., FESSELE, S., JACOBI, C. A., SCHALLER, M., NELSON, P. J., AUTENRIETH, I. B. (2000). Yersinia enterocolitica invasin protein triggers IL-8 production in epithelial cells via activation of Rel p65-p65 homodimers. FASEB J. 14: 1471-1484 [Abstract] [Full Text]  
  • Kampik, D., Schulte, R., Autenrieth, I. B. (2000). Yersinia enterocolitica Invasin Protein Triggers Differential Production of Interleukin-1, Interleukin-8, Monocyte Chemoattractant Protein 1, Granulocyte-Macrophage Colony-Stimulating Factor, and Tumor Necrosis Factor Alpha in Epithelial Cells: Implications for Understanding the Early Cytokine Network in Yersinia Infections. Infect. Immun. 68: 2484-2492 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»