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Infect Immun, March 1998, p. 907-911, Vol. 66, No. 3
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

D-Lactate Production and [¹⁴C]Succinic Acid Uptake by Adherent and Nonadherent Escherichia coli

K. McCabe, M. D. Mann,^* and M. D. Bowie

Institute of Child Health, University of Cape Town, Red Cross War Memorial Children's Hospital, Rondebosch 7700, South Africa

Received 15 May 1997/Returned for modification 15 July 1997/Accepted 11 December 1997

Escherichia coli isolates of different adherence phenotypes produced different amounts of D-lactate. Alterations of culture conditions did not influence the amount of D-lactate produced. The observed pH decreases in tissue culture medium corresponded with increases in D-lactate concentration. Very little [¹⁴C]succinic acid was incorporated into cells during the in vitro incubation of adherent and nonadherent E. coli with HeLa cells, but the amounts of tracer removed from the culture medium by adherent and nonadherent strains differed. The results are further evidence of a difference in the metabolic behavior of adherent and nonadherent E. coli.

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^* Corresponding author. Mailing address: Department of Paediatrics and Child Health, Red Cross Children's Hospital, Klipfontein Rd., Rondebosch 7700, South Africa. Phone: 27-21-658-5302. Fax: 27-21-689-1287. E-mail: mmann{at}ich.uct.ac.za.

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