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Infect Immun, March 1998, p. 938-943, Vol. 66, No. 3
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Mutation in the peb1A Locus of Campylobacter jejuni Reduces Interactions with Epithelial Cells and Intestinal Colonization of Mice

Zhiheng Pei,1 Christophe Burucoa,2 Bernadette Grignon,2 Shahida Baqar,3 Xiao-Zhe Huang,4 Dennis J. Kopecko,4 A. L. Bourgeois,3 Jean-Louis Fauchere,2 and Martin J. Blaser1,*

Departments of Medicine and Microbiology and Immunology, Vanderbilt University School of Medicine and Veterans Affairs Medical Center, Nashville, Tennessee1; Laboratoire de Microbiologie A, CHU la Miletrie, 86021 Poitiers, France2; Enteric Disease Program, Naval Medical Research Institute, Bethesda, Maryland3; and Laboratory of Enteric and Sexually Transmitted Diseases, FDA-CBER, Rockville, Maryland4

Received 11 July 1997/Returned for modification 1 October 1997/Accepted 8 December 1997

Campylobacter jejuni is one of the leading causes of bacterial diarrhea throughout the world. We previously found that PEB1 is a homolog of cluster 3 binding proteins of bacterial ABC transporters and that a C. jejuni adhesin, cell-binding factor 1 (CBF1), if not identical to, contains PEB1. A single protein migrating at approximately 27 to 28 kDa was recognized by anti-CBF1 and anti-PEB1. To determine the role that the operon encoding PEB1 plays in C. jejuni adherence, peb1A, the gene encoding PEB1, was disrupted in strain 81-176 by insertion of a kanamycin resistance gene through homologous recombination. Inactivation of this operon completely abolished expression of CBF1, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. In comparison to the wild-type strain, the mutant strain showed 50- to 100-fold less adherence to and 15-fold less invasion of epithelial cells in culture. Mouse challenge studies showed that the rate and duration of intestinal colonization by the mutant were significantly lower and shorter than with the wild-type strain. In summary, PEB1 is identical to a previously identified cell-binding factor, CBF1, in C. jejuni, and the peb1A locus plays an important role in epithelial cell interactions and in intestinal colonization in a mouse model.

* Corresponding author. Mailing address: Division of Infectious Diseases, A-3310 Medical Center North, Vanderbilt University School of Medicine, Nashville, TN 37232. Phone: (615) 322-2035. Fax: (615) 343-6160. E-mail: Martin.Blaser{at}mcmail.vanderbilt.edu.




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