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Infect Immun, April 1998, p. 1507-1512, Vol. 66, No. 4
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Inhibition of the Production of Anti-OspA Borreliacidal Antibody with T Cells from Hamsters Vaccinated against Borrelia burgdorferi

Jani R. Jensen,^1,2,3 Brian K. Du Chateau,^1,2,4 Erik L. Munson,^1,2 Steven M. Callister,^3,5 and Ronald F. Schell^1,2,4,*

Wisconsin State Laboratory of Hygiene¹ and Departments of Medical Microbiology and Immunology² and Bacteriology,⁴ University of Wisconsin, Madison, Wisconsin 53706, and Microbiology Research Laboratory³ and Department of Infectious Diseases,⁵ Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601

Received 19 September 1997/Returned for modification 25 November 1997/Accepted 20 January 1998

The serious morbidity associated with Lyme borreliosis has focused considerable effort on the development of a comprehensive vaccine for protection against infection with Borrelia burgdorferi. Induction of borreliacidal antibody by vaccination or infection has been shown to correlate with protection of humans and animals against infection with the Lyme spirochete. In this report, we showed that high levels of borreliacidal antibody (titer of 1,280) were produced in vitro when T and B cells from hamsters 14 days after vaccination were incubated with macrophages and B. burgdorferi. By contrast, T and B cells from hamsters 7 or 21 days after vaccination failed to initiate production of borreliacidal activity. Furthermore, the T cells from hamsters 7 or 21 days after vaccination inhibited the in vitro production of borreliacidal antibody when cocultured with T and B cells obtained from hamsters 14 days after vaccination. When cell-free supernatants from the suspensions of T and B cells from hamsters 14 days after vaccination were absorbed with recombinant OspA, they lost nearly all borreliacidal activity. The removal of anti-OspA antibody resulted in a decrease in borreliacidal titer from 1,280 to less than 4. These results demonstrate that T cells from vaccinated animals can prevent a sustained production of protective borreliacidal antibody.

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^* Corresponding author. Mailing address: University of Wisconsin, Wisconsin State Laboratory of Hygiene, 465 Henry Mall, Madison, WI 53706. Phone: (608) 262-3634. Fax: (608) 265-3451.

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