This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Swanson, A. F.
Right arrow Articles by Kuo, C.-c.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Swanson, A. F.
Right arrow Articles by Kuo, C.-c.

 Previous Article  |  Next Article 

Infect Immun, April 1998, p. 1607-1612, Vol. 66, No. 4
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Human Mannose-Binding Protein Inhibits Infection of HeLa Cells by Chlamydia trachomatis

Albertina F. Swanson,1 R. Alan B. Ezekowitz,2 Amy Lee,1 and Cho-chou Kuo1,*

Department of Pathobiology, University of Washington, Seattle, Washington 98195,1 and Department of Pediatrics, Harvard Medical School, Pediatric Service, Massachusetts General Hospital, Boston, Massachusetts 021142

Received 5 November 1997/Returned for modification 6 January 1998/Accepted 27 January 1998

The role that collectin (mannose-binding protein) may play in the host's defense against chlamydial infection was investigated. Recombinant human mannose-binding protein was used in the inhibition of cell culture infection by Chlamydia trachomatis (C/TW-3/OT, E/UW-5/Cx, and L2/434/Bu), Chlamydia pneumoniae (AR-39), and Chlamydia psittaci (6BC). Mannose-binding protein (MBP) inhibited infection of all chlamydial strains by at least 50% at 0.098 µg/ml for TW-3 and UW-5, and at 6.25 µg/ml for 434, AR-39, and 6BC. The ability of MBP to inhibit infection with strain L2 was not affected by supplementation with complement or addition of an L2-specific neutralizing monoclonal antibody. Enzyme-linked immunosorbent assay and dot blot analyses showed MBP bound to the surface of the organism to exert inhibition, which appeared to block the attachment of radiolabeled organisms to HeLa cells. Immunoblotting and affinity chromatography indicated that MBP binds to the 40-kDa glycoprotein (the major outer membrane protein) on the outer surface of the chlamydial elementary body. Hapten inhibition assays with monosaccharides and defined oligosaccharides showed that the inhibitory effects of MBP were abrogated by mannose or high-mannose type oligomannose-oligosaccharide. The latter carbohydrate is the ligand of the 40-kDa glycoprotein of C. trachomatis L2, which is known to mediate attachment, suggesting that the MBP binds to high mannose moieties on the surface of chlamydial organisms. These results suggest that MBP plays a role in first-line host defense against chlamydial infection in humans.

* Corresponding author. Mailing address: Department of Pathobiology, Box 357238, University of Washington, Seattle, WA 98195. Phone: (206) 543-8689. Fax: (206) 543-3873. E-mail: cckuo{at}u.washington.edu.




This article has been cited by other articles:

  • Oberley, R. E., Goss, K. L., Hoffmann, D. S., Ault, K. A., Neff, T. L., Ramsey, K. H., Snyder, J. M. (2007). Regulation of surfactant protein D in the mouse female reproductive tract in vivo. Mol Hum Reprod 13: 863-868 [Abstract] [Full Text]  
  • Al-Mously, N., Eley, A. (2007). Interaction of Chlamydia trachomatis serovar E with male genital tract epithelium results in secretion of proinflammatory cytokines. J Med Microbiol 56: 1025-1032 [Abstract] [Full Text]  
  • Sziller, I., Babula, O., Ujhazy, A., Nagy, B., Hupuczi, P., Papp, Z., Linhares, I.M., Ledger, W.J., Witkin, S.S. (2007). Chlamydia trachomatis infection, Fallopian tube damage and a mannose-binding lectin codon 54 gene polymorphism. Hum Reprod 22: 1861-1865 [Abstract] [Full Text]  
  • Saevarsdottir, S., Oskarsson, O. O., Aspelund, T., Eiriksdottir, G., Vikingsdottir, T., Gudnason, V., Valdimarsson, H. (2005). Mannan binding lectin as an adjunct to risk assessment for myocardial infarction in individuals with enhanced risk. JEM 201: 117-125 [Abstract] [Full Text]  
  • Oberley, R. E., Goss, K. L., Ault, K. A., Crouch, E. C., Snyder, J. M. (2004). Surfactant protein D is present in the human female reproductive tract and inhibits Chlamydia trachomatis infection. Mol Hum Reprod 10: 861-870 [Abstract] [Full Text]  
  • Spence, J. D., Norris, J. (2003). Infection, Inflammation, and Atherosclerosis. Stroke 34: 333-334 [Full Text]  
  • Rugonfalvi-Kiss, S., Endresz, V., Madsen, H. O., Burian, K., Duba, J., Prohaszka, Z., Karadi, I., Romics, L., Gonczol, E., Fust, G., Garred, P. (2002). Association of Chlamydia pneumoniae With Coronary Artery Disease and Its Progression Is Dependent on the Modifying Effect of Mannose-Binding Lectin. Circulation 106: 1071-1076 [Abstract] [Full Text]  
  • Gulati, S., Sastry, K., Jensenius, J. C., Rice, P. A., Ram, S. (2002). Regulation of the Mannan-Binding Lectin Pathway of Complement on Neisseria gonorrhoeae by C1-Inhibitor and {alpha}2-Macroglobulin. J. Immunol. 168: 4078-4086 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»