This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ben-Ami, G.
Right arrow Articles by Rosenshine, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ben-Ami, G.
Right arrow Articles by Rosenshine, I.

 Previous Article  |  Next Article 

Infect Immun, April 1998, p. 1755-1758, Vol. 66, No. 4
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Agents That Inhibit Rho, Rac, and Cdc42 Do Not Block Formation of Actin Pedestals in HeLa Cells Infected with Enteropathogenic Escherichia coli

Goni Ben-Ami,1 Vered Ozeri,2 Emanuel Hanski,2 Fred Hofmann,3 Klaus Aktories,3 Klaus M. Hahn,4 Gary M. Bokoch,4,5 and Ilan Rosenshine1,*

Departments of Molecular Genetics and Biotechnology1 and of Clinical Microbiology,2 Faculty of Medicine, The Hebrew University, Jerusalem 91120, Israel; Institute of Pharmacology and Toxicology, Albert-Ludwigs University, Freiburg, Germany3; and Departments of Cell Biology4 and of Immunology,5 The Scripps Research Institute, La Jolla, California 92037

Received 31 October 1997/Returned for modification 6 January 1998/Accepted 20 January 1998

Enteropathogenic Escherichia coli (EPEC) induces formation of actin pedestals in infected host cells. Agents that inhibit the activity of Rho, Rac, and Cdc42, including Clostridium difficile toxin B (ToxB), compactin, and dominant negative Rho, Rac, and Cdc42, did not inhibit formation of actin pedestals. In contrast, treatment of HeLa cells with ToxB inhibited EPEC invasion. Thus, Rho, Rac, and Cdc42 are not required for assembly of actin pedestals; however, they may be involved in EPEC uptake by HeLa cells.

* Corresponding author. Mailing address: Department of Molecular Genetics and Biotechnology, The Hebrew University Faculty of Medicine, Room 119 International Building, Ein Karem, P.O.B. 12272, Jerusalem 91120, Israel. Phone: 972 2 6758754. Fax: 972 2 6757308. E-mail: ilanro{at}cc.huji.ac.il.




This article has been cited by other articles:

  • Brown, M. D., Bry, L., Li, Z., Sacks, D. B. (2008). Actin Pedestal Formation by Enteropathogenic Escherichia coli Is Regulated by IQGAP1, Calcium, and Calmodulin. J. Biol. Chem. 283: 35212-35222 [Abstract] [Full Text]  
  • Peralta-Ramirez, J., Hernandez, J. M., Manning-Cela, R., Luna-Munoz, J., Garcia-Tovar, C., Nougayrede, J.-P., Oswald, E., Navarro-Garcia, F. (2008). EspF Interacts with Nucleation-Promoting Factors To Recruit Junctional Proteins into Pedestals for Pedestal Maturation and Disruption of Paracellular Permeability. Infect. Immun. 76: 3854-3868 [Abstract] [Full Text]  
  • Shifrin, Y., Peleg, A., Ilan, O., Nadler, C., Kobi, S., Baruch, K., Yerushalmi, G., Berdichevsky, T., Altuvia, S., Elgrably-Weiss, M., Abe, C., Knutton, S., Sasakawa, C., Ritchie, J. M., Waldor, M. K., Rosenshine, I. (2008). Transient Shielding of Intimin and the Type III Secretion System of Enterohemorrhagic and Enteropathogenic Escherichia coli by a Group 4 Capsule. J. Bacteriol. 190: 5063-5074 [Abstract] [Full Text]  
  • Hardwidge, P. R., Rodriguez-Escudero, I., Goode, D., Donohoe, S., Eng, J., Goodlett, D. R., Aebersold, R., Finlay, B. B. (2004). Proteomic Analysis of the Intestinal Epithelial Cell Response to Enteropathogenic Escherichia coli. J. Biol. Chem. 279: 20127-20136 [Abstract] [Full Text]  
  • Hopkins, A. M., Walsh, S. V., Verkade, P., Boquet, P., Nusrat, A. (2003). Constitutive activation of Rho proteins by CNF-1 influences tight junction structure and epithelial barrier function. J. Cell Sci. 116: 725-742 [Abstract] [Full Text]  
  • Gubina, E., Chen, T., Zhang, L., Lizzio, E. F., Kozlowski, S. (2002). CD43 polarization in unprimed T cells can be dissociated from raft coalescence by inhibition of HMG CoA reductase. Blood 99: 2518-2525 [Abstract] [Full Text]  
  • Akeda, Y., Kodama, T., Kashimoto, T., Cantarelli, V., Horiguchi, Y., Nagayama, K., Iida, T., Honda, T. (2002). Dominant-Negative Rho, Rac, and Cdc42 Facilitate the Invasion Process of Vibrio parahaemolyticus into Caco-2 Cells. Infect. Immun. 70: 970-973 [Abstract] [Full Text]  
  • Simonovic, I., Arpin, M., Koutsouris, A., Falk-Krzesinski, H. J., Hecht, G. (2001). Enteropathogenic Escherichia coli Activates Ezrin, Which Participates in Disruption of Tight Junction Barrier Function. Infect. Immun. 69: 5679-5688 [Abstract] [Full Text]  
  • Goosney, D. L., DeVinney, R., Finlay, B. B. (2001). Recruitment of Cytoskeletal and Signaling Proteins to Enteropathogenic and Enterohemorrhagic Escherichia coli Pedestals. Infect. Immun. 69: 3315-3322 [Abstract] [Full Text]  
  • Czerucka, D., Dahan, S., Mograbi, B., Rossi, B., Rampal, P. (2001). Implication of Mitogen-Activated Protein Kinases in T84 Cell Responses to Enteropathogenic Escherichia coli Infection. Infect. Immun. 69: 1298-1305 [Abstract] [Full Text]  
  • Vallance, B. A., Finlay, B. B. (2000). Exploitation of host cells by enteropathogenic Escherichiacoli. Proc. Natl. Acad. Sci. USA 97: 8799-8806 [Abstract] [Full Text]  
  • Phillips, A. D., Giròn, J., Hicks, S., Dougan, G., Frankel, G. (2000). Intimin from enteropathogenic Escherichia coli mediates remodelling of the eukaryotic cell surface. Microbiology 146: 1333-1344 [Abstract] [Full Text]  
  • Johnson, D. I. (1999). Cdc42: An Essential Rho-Type GTPase Controlling Eukaryotic Cell Polarity. Microbiol. Mol. Biol. Rev. 63: 54-105 [Abstract] [Full Text]  
  • Mounier, J, Laurent, V, Hall, A, Fort, P, Carlier, M., Sansonetti, P., Egile, C (1999). Rho family GTPases control entry of Shigella flexneri into epithelial cells but not intracellular motility. J. Cell Sci. 112: 2069-2080 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»