Expression of the Candida albicans Gene ALS1 in Saccharomyces cerevisiae Induces Adherence to Endothelial and Epithelial Cells

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Infect Immun, April 1998, p. 1783-1786, Vol. 66, No. 4
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Expression of the Candida albicans Gene ALS1 in Saccharomyces cerevisiae Induces Adherence to Endothelial and Epithelial Cells

Yue Fu,¹ Günter Rieg,¹ William A. Fonzi,² Paul H. Belanger,¹ John E. Edwards Jr.,¹^,³ and Scott G. Filler¹^,³^,^*

St. John's Cardiovascular Research Center, Division of Infectious Diseases, Department of Medicine, Harbor-UCLA Research and Education Institute, Torrance, California 90502¹; Department of Microbiology and Immunology, Georgetown University, Washington, D.C. 20007²; and UCLA School of Medicine, Los Angeles, California 90024³

Received 13 November 1997/Returned for modification 8 December 1997/Accepted 15 January 1998

To identify genes encoding adhesins that mediate the binding of Candida albicans to endothelial cells, a genomic library fromthis organism was constructed and used to transform Saccharomycescerevisiae. These transformed organisms were screened for adherenceto endothelial cells, and a highly adherent clone was identified.The adherence of this clone to endothelial cells was over 100-foldgreater than that of control S. cerevisiae transformed with theempty plasmid. This clone also exhibited enhanced adherence toepithelial cells. The C. albicans gene contained within this clonewas found to be ALS1. These results indicate that ALS1 may encodea candidal adhesin.

^* Corresponding author. Mailing address: Division of Infectious Diseases, Harbor-UCLA Research and Education Institute, Bldg.RB-2, 1124 West Carson St., Torrance, CA 90502. Phone: (310) 222-6426.Fax: (310) 782-2016. E-mail: Filler{at}AFP76.HUMC.EDU.

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