This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An author's correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van der Ende, A.
Right arrow Articles by Dankert, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van der Ende, A.
Right arrow Articles by Dankert, J.

 Previous Article  |  Next Article 

Infect Immun, May 1998, p. 1822-1826, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

cagA-Positive Helicobacter pylori Populations in China and The Netherlands Are Distinct

Arie van der Ende,1,* Zhi-Jun Pan,1,2,dagger Aldert Bart,1 René W. M. van der Hulst,3 Monique Feller,1 Shu-Dong Xiao,2 Guido N. J. Tytgat,3 and Jacob Dankert1

Departments of Medical Microbiology1 and Gastroenterology,3 Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands, and Shanghai Institute of Digestive Disease, Shanghai Second Medical University, Shanghai, People's Republic of China2

Received 26 November 1997/Returned for modification 23 January 1998/Accepted 11 February 1998

The aim of this research was to study whether and to what extent Chinese cagA-positive Helicobacter pylori isolates differ from those in The Netherlands. Analysis of random amplified polymorphic DNA (RAPD)-PCR-assessed DNA fingerprints of chromosomal DNA of 24 cagA-positive H. pylori isolates from Dutch (n = 12) and Chinese (n = 10) patients yielded the absence of clustering. Based on comparison of the sequence of a 243-nucleotide part of cagA, the Dutch (group I) and Chinese (group II) H. pylori isolates formed two separate branches with high confidence limits in the phylogenetic tree. These two clusters were not observed when the sequence of a 240-bp part of glmM was used in the comparison. The number of nonsynonymous substitutions was much higher in cagA than in glmM, indicating positive selection. The average levels of divergence of cagA at the nucleotide and protein levels between group I and II isolates were found to be high, 13.3 and 17.9%, respectively. Possibly, the pathogenicity island (PAI) that has been integrated into the chromosome of the ancestor of H. pylori now circulating in China contained a different cagA than the PAI that has been integrated into the chromosome of the ancestor of H. pylori now circulating in The Netherlands. We conclude that in China and The Netherlands, two distinct cagA-positive H. pylori populations are circulating.

* Corresponding author. Mailing address: Academic Medical Center, Department of Medical Microbiology, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands. Phone: 31-20-5664862. Fax: 31-20-6979271. E-mail: a.vanderende{at}amc.uva.nl.

dagger Present address: Department of Molecular Biology, School of Medicine, Washington University, St. Louis, MO 63110.

Infect Immun, May 1998, p. 1822-1826, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Owen, R. J., Xerry, J. (2007). Geographical conservation of short inserts in the signal and middle regions of the Helicobacter pylori vacuolating cytotoxin gene. Microbiology 153: 1176-1186 [Abstract] [Full Text]  
  • Kersulyte, D., Kalia, A., Zhang, M., Lee, H.-K., Subramaniam, D., Kiuduliene, L., Chalkauskas, H., Berg, D. E. (2004). Sequence Organization and Insertion Specificity of the Novel Chimeric ISHp609 Transposable Element of Helicobacter pylori. J. Bacteriol. 186: 7521-7528 [Abstract] [Full Text]  
  • Lee, K.-H., Cho, M.-J., Yamaoka, Y., Graham, D. Y., Yun, Y.-J., Woo, S.-Y., Lim, C.-Y., Ko, K.-S., Kim, B.-J., Jung, H.-C., Lee, W.-K., Rhee, K.-H., Kook, Y.-H. (2004). Alanine-Threonine Polymorphism of Helicobacter pylori RpoB Is Correlated with Differential Induction of Interleukin-8 in MKN45 Cells. J. Clin. Microbiol. 42: 3518-3524 [Abstract] [Full Text]  
  • Dailidiene, D., Dailide, G., Ogura, K., Zhang, M., Mukhopadhyay, A. K., Eaton, K. A., Cattoli, G., Kusters, J. G., Berg, D. E. (2004). Helicobacter acinonychis: Genetic and Rodent Infection Studies of a Helicobacter pylori-Like Gastric Pathogen of Cheetahs and Other Big Cats. J. Bacteriol. 186: 356-365 [Abstract] [Full Text]  
  • Owen, R. J., Xerry, J., Gotada, T., Naylor, G., Tompkins, D. (2004). Analysis of geospecific markers for Helicobacter pylori variants in patients from Japan and Nigeria by triple-locus nucleotide sequence typing. Microbiology 150: 151-161 [Abstract] [Full Text]  
  • Datta, S., Chattopadhyay, S., Balakrish Nair, G., Mukhopadhyay, A. K., Hembram, J., Berg, D. E., Rani Saha, D., Khan, A., Santra, A., Bhattacharya, S. K., Chowdhury, A. (2003). Virulence Genes and Neutral DNA Markers of Helicobacter pylori Isolates from Different Ethnic Communities of West Bengal, India. J. Clin. Microbiol. 41: 3737-3743 [Abstract] [Full Text]  
  • Rahman, M., Mukhopadhyay, A. K., Nahar, S., Datta, S., Mashhud Ahmad, M., Sarker, S., Masud, I. M., Engstrand, L., Albert, M. J., Nair, G. B., Berg, D. E. (2003). DNA-Level Characterization of Helicobacter pylori Strains from Patients with Overt Disease and with Benign Infections in Bangladesh. J. Clin. Microbiol. 41: 2008-2014 [Abstract] [Full Text]  
  • Takata, T., Aras, R., Tavakoli, D., Ando, T., Olivares, A. Z., Blaser, M. J. (2002). Phenotypic and genotypic variation in methylases involved in type II restriction-modification systems in Helicobacter pylori. Nucleic Acids Res 30: 2444-2452 [Abstract] [Full Text]  
  • Kersulyte, D., Velapatino, B., Dailide, G., Mukhopadhyay, A. K., Ito, Y., Cahuayme, L., Parkinson, A. J., Gilman, R. H., Berg, D. E. (2002). Transposable Element ISHp608 of Helicobacter pylori: Nonrandom Geographic Distribution, Functional Organization, and Insertion Specificity. J. Bacteriol. 184: 992-1002 [Abstract] [Full Text]  
  • Ando, T., Peek, R. M. Jr., Lee, Y.-C., Krishna, U., Kusugami, K., Blaser, M. J. (2002). Host Cell Responses to Genotypically Similar Helicobacter pylori Isolates from United States and Japan. CVI 9: 167-175 [Abstract] [Full Text]  
  • Hynes, S. O., Broutet, N., Eurohepygast Study Group, , Wadstrom, T., Mikelsaar, M., O'Toole, P. W., Telford, J., Engstrand, L., Kamiya, S., Mentis, A. F., Moran, A. P. (2002). Phenotypic Variation of Helicobacter pylori Isolates from Geographically Distinct Regions Detected by Lectin Typing. J. Clin. Microbiol. 40: 227-232 [Abstract] [Full Text]  
  • Ando, T., Peek, R. M., Pride, D., Levine, S. M., Takata, T., Lee, Y.-C., Kusugami, K., van der Ende, A., Kuipers, E. J., Kusters, J. G., Blaser, M. J. (2002). Polymorphisms of Helicobacter pylori HP0638 Reflect Geographic Origin and Correlate with cagA Status. J. Clin. Microbiol. 40: 239-246 [Abstract] [Full Text]  
  • Maggi Solca, N., Bernasconi, M. V., Valsangiacomo, C., Van Doorn, L.-J., Piffaretti, J.-C. (2001). Population genetics of Helicobacter pylori in the southern part of Switzerland analysed by sequencing of four housekeeping genes (atpD, glnA, scoB and recA), and by vacA, cagA, iceA and IS605 genotyping. Microbiology 147: 1693-1707 [Abstract] [Full Text]  
  • Figueiredo, C., Quint, W., Nouhan, N., van den Munckhof, H., Herbrink, P., Scherpenisse, J., de Boer, W., Schneeberger, P., Perez-Perez, G., Blaser, M. J., van Doorn, L.-J. (2001). Assessment of Helicobacter pylori vacA and cagA Genotypes and Host Serological Response. J. Clin. Microbiol. 39: 1339-1344 [Abstract] [Full Text]  
  • Rota, C. A., Pereira-Lima, J. C., Blaya, C., Nardi, N. B. (2001). Consensus and Variable Region PCR Analysis of Helicobacter pylori 3' Region of cagA Gene in Isolates from Individuals with or without Peptic Ulcer. J. Clin. Microbiol. 39: 606-612 [Abstract] [Full Text]  
  • Han, S.-R., Zschausch, H.-C. E., Meyer, H.-G. W., Schneider, T., Loos, M., Bhakdi, S., Maeurer, M. J. (2000). Helicobacter pylori: Clonal Population Structure and Restricted Transmission within Families Revealed by Molecular Typing. J. Clin. Microbiol. 38: 3646-3651 [Abstract] [Full Text]  
  • Kersulyte, D., Mukhopadhyay, A. K., Velapatiño, B., Su, W., Pan, Z., Garcia, C., Hernandez, V., Valdez, Y., Mistry, R. S., Gilman, R. H., Yuan, Y., Gao, H., Alarcón, T., López-Brea, M., Balakrish Nair, G., Chowdhury, A., Datta, S., Shirai, M., Nakazawa, T., Ally, R., Segal, I., Wong, B. C. Y., Lam, S. K., Olfat, F. O., Borén, T., Engstrand, L., Torres, O., Schneider, R., Thomas, J. E., Czinn, S., Berg, D. E. (2000). Differences in Genotypes of Helicobacter pylori from Different Human Populations. J. Bacteriol. 182: 3210-3218 [Abstract] [Full Text]  
  • Mukhopadhyay, A. K., Kersulyte, D., Jeong, J.-Y., Datta, S., Ito, Y., Chowdhury, A., Chowdhury, S., Santra, A., Bhattacharya, S. K., Azuma, T., Nair, G. B., Berg, D. E. (2000). Distinctiveness of Genotypes of Helicobacter pylori in Calcutta, India. J. Bacteriol. 182: 3219-3227 [Abstract] [Full Text]  
  • Kimmel, B., Bosserhoff, A., Frank, R., Gross, R., Goebel, W., Beier, D. (2000). Identification of Immunodominant Antigens from Helicobacter pylori and Evaluation of Their Reactivities with Sera from Patients with Different Gastroduodenal Pathologies. Infect. Immun. 68: 915-920 [Abstract] [Full Text]  
  • Ito, Y., Azuma, T., Ito, S., Suto, H., Miyaji, H., Yamazaki, Y., Kato, T., Kohli, Y., Keida, Y., Kuriyama, M. (2000). Sequence Analysis and Clinical Significance of the iceA Gene from Helicobacter pylori Strains in Japan. J. Clin. Microbiol. 38: 483-488 [Abstract] [Full Text]  
  • Tanahashi, T., Kita, M., Kodama, T., Sawai, N., Yamaoka, Y., Mitsufuji, S., Katoh, F., Imanishi, J. (2000). Comparison of PCR-Restriction Fragment Length Polymorphism Analysis and PCR-Direct Sequencing Methods for Differentiating Helicobacter pylori ureB Gene Variants. J. Clin. Microbiol. 38: 165-169 [Abstract] [Full Text]  
  • van Doorn, L.-J., Figueiredo, C., Sanna, R., Blaser, M. J., Quint, W. G. V. (1999). Distinct Variants of Helicobacter pylori cagA Are Associated with vacA Subtypes. J. Clin. Microbiol. 37: 2306-2311 [Abstract] [Full Text]  
  • Covacci, A., Telford, J. L., Giudice, G. D., Parsonnet, J., Rappuoli, R. (1999). Helicobacter pylori Virulence and Genetic Geography. Science 284: 1328-1333 [Abstract] [Full Text]  
  • Ng, E. K. W., Thompson, S. A., Perez-Perez, G. I., Kansau, I., van der Ende, A., Labigne, A., Sung, J. J. Y., Chung, S. C. S., Blaser, M. J. (1999). Helicobacter pylori Heat Shock Protein A: Serologic Responses and Genetic Diversity. CVI 6: 377-382 [Abstract] [Full Text]  
  • Evans, D. G., Queiroz, D. M. M., Mendes, E. N., Evans, D. J. Jr. (1998). Helicobacter pylori cagA Status and s and m Alleles of vacA in Isolates from Individuals with a Variety of H. pylori-Associated Gastric Diseases. J. Clin. Microbiol. 36: 3435-3437 [Abstract] [Full Text]  
  • Suerbaum, S., Smith, J. M., Bapumia, K., Morelli, G., Smith, N. H., Kunstmann, E., Dyrek, I., Achtman, M. (1998). Free recombination within Helicobacter pylori. Proc. Natl. Acad. Sci. USA 95: 12619-12624 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»