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Infect Immun, May 1998, p. 1869-1877, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Cytopathic Effects of the Major Surface Protein and
the Chymotrypsinlike Protease of Treponema denticola
J. Christopher
Fenno,1
Pauline M.
Hannam,1
W. Keung
Leung,2,
Muneaki
Tamura,1
Veli-Jukka
Uitto,2 and
Barry C.
McBride1,2,*
Department of Microbiology and
Immunology1 and
Department of Oral
Biological and Medical Sciences,2 University
of British Columbia, Vancouver, Canada
Received 8 July 1997/Returned for modification 26 September
1997/Accepted 15 January 1998
Prominent antigens of Treponema denticola have been
suggested to be mediators of the cytopathic effects typically seen in periodontal disease. In the present study of the T. denticola major surface protein (Msp) and the surface-expressed
chymotrypsinlike protease complex (CTLP), we characterized the ability
of these proteins to adhere to and lyse epithelial cells. Msp and CTLP were closely associated in spirochete outer membranes. Purified Msp,
both native and recombinant, and CTLP bound to glutaraldehyde-fixed periodontal ligament epithelial cells. Adherence of Msp was partially blocked by specific antibodies. Adherence of CTLP was partially blocked
by serine protease inhibitors and was further inhibited by specific
antibodies. Both native Msp and CTLP were cytotoxic toward periodontal
ligament epithelial cells, and their cytotoxicity was inhibited by the
same treatments that inhibited adherence. Msp, but not CTLP, lysed
erythrocytes. Msp complex (partially purified outer membranes free of
protease activity) was cytotoxic toward a variety of different cell
types. Pore-forming activities of recombinant Msp in black lipid model
membrane assays and in HeLa cell membranes were similar to those
reported for the native protein, supporting the hypothesis that Msp
cytotoxicity was due to its pore-forming activity.
*
Corresponding author. Mailing address: Faculty of
Science, University of British Columbia, 6270 University Blvd.,
Vancouver, B.C. V6T 1Z4, Canada. Phone: (604) 822-3337. Fax: (604)
822-0677. E-mail: mcbride{at}unixg.ubc.ca.

Present address: Department of Periodontology, University of Hong
Kong, 3/F Prince Philip Dental Hospital, Hong Kong.
Infect Immun, May 1998, p. 1869-1877, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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