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Infect Immun, May 1998, p. 1946-1952, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Integrins
v
3 and
5
1 Mediate Attachment of Lyme Disease
Spirochetes to Human Cells
Jenifer
Coburn,1,*
Loranne
Magoun,2
Sarah C.
Bodary,3 and
John M.
Leong2
Division of Rheumatology and Immunology,
Tufts-New England Medical Center, Boston, Massachusetts
021111;
Department of Molecular Genetics
and Microbiology, University of Massachusetts Medical Center,
Worcester, Massachusetts 016552; and
Department of Immunology, Genentech, Inc., South San
Francisco, California 940803
Received 17 October 1997/Returned for modification 8 December
1997/Accepted 20 February 1998
Borrelia burgdorferi (sensu lato), the agent of Lyme
disease, is able to cause chronic, multisystemic infections in human and animal hosts. Attachment of the spirochete to host cells is likely
to be important for the colonization of diverse tissues. The
platelet-specific integrin
IIb
3 was
previously identified as a receptor for all three species of Lyme
disease spirochetes (B. burgdorferi sensu stricto, B. garinii, and B. afzelii). Here we show that B. burgdorferi also recognizes the widely expressed integrins
v
3 and
5
1,
known as the vitronectin and fibronectin receptors, respectively. Three
representatives of each species of Lyme disease spirochete were tested
for the ability to bind to purified
v
3
and
5
1. All of the strains tested bound
to at least one integrin. Binding to one integrin was not always predictive of binding to other integrins, and several different integrin preference profiles were identified. Attachment of the infectious B. burgdorferi strain N40 to purified
v
3 and
5
1 was inhibited by RGD peptides and the appropriate receptor-specific antibodies. Binding to
v
3 was also shown
by using a transfected cell line that expresses this receptor but not
IIb
3. Attachment of B. burgdorferi N40 to human erythroleukemia cells and to human saphenous vein endothelial cells was mediated by both
5
1 and
v
3.
Our results show that multiple integrins mediate attachment of Lyme
disease spirochetes to host cells.
*
Corresponding author. Mailing address: Division of
Rheumatology and Immunology, Tufts-New England Medical Center, Box 406, 750 Washington St., Boston, MA 02111. Phone: (617) 636-5952. Fax: (617)
636-4252. E-mail: jcoburn_bor{at}opal.tufts.edu.
Infect Immun, May 1998, p. 1946-1952, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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