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Infect Immun, May 1998, p. 2026-2032, Vol. 66, No. 5
Channing Laboratory,
Received 22 October 1997/Returned for modification 26 November
1997/Accepted 2 February 1998
We have developed an adoptive cell transfer model in mice to study
the ability of a glycoprotein conjugate vaccine to induce immunologic
memory for the polysaccharide moiety. We used type III capsular
polysaccharide from the clinically relevant pathogen group B
streptococci conjugated to tetanus toxoid (GBSIII-TT) as our model
vaccine. GBS are a major cause of neonatal infections in humans, and
type-specific antibodies to the capsular polysaccharide protect against
invasive disease. Adoptive transfer of splenocytes from mice immunized
with the GBSIII-TT conjugate vaccine conferred anti-polysaccharide
immunologic memory to naive recipient mice. The transfer of memory
occurred in a dose-dependent manner. The observed anamnestic immune
response was characterized by (i) more rapid kinetics, (ii) isotype
switching from immunoglobulin M (IgM) to IgG, and (iii) 10-fold-higher
levels of type III-specific IgG antibody than for the primary response
in animals with cells transferred from placebo-immunized mice. The
adoptive cell transfer model described in this paper can be used for at
least two purposes: (i) to evaluate conjugate vaccines with different
physicochemical properties for their ability to induce immunologic
memory and (ii) to study the cellular interactions required for an
immune response to these molecules.
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Immunologic Memory Induced by a Glycoconjugate
Vaccine in a Murine Adoptive Lymphocyte Transfer Model
*
Corresponding author. Mailing address: Channing
Laboratory, 181 Longwood Ave., Boston, MA 02115. Phone: (617) 525-2192. Fax: (617) 731-1541. E-mail:
hilde-kari.guttormsen{at}channing.harvard.edu.
Infect Immun, May 1998, p. 2026-2032, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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