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Infect Immun, May 1998, p. 2052-2059, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Cell Wall-Associated Glyceraldehyde-3-Phosphate Dehydrogenase of Candida albicans Is Also a Fibronectin and Laminin Binding Protein

Daniel Gozalbo,1 Inés Gil-Navarro,1 Inmaculada Azorín,2 Jaime Renau-Piqueras,2 José P. Martínez,1 and M. Luisa Gil1,*

Departamento de Microbiología y Ecología, Facultad de Farmacia, Universitat de València,1 and Sección de Biología y Patología Celular, Centro de Investigación, Hospital La Fe de Valencia,2 Valencia, Spain

Received 3 December 1997/Returned for modification 13 January 1998/Accepted 25 February 1998

By immunoelectron microscopy with a polyclonal antibody against the cytosolic glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Candida albicans (anti-GAPDH PAb), the protein was clearly detected at the outer surface of the cell wall, particularly on blastoconidia, as well as in the cytoplasm. Intact blastoconidia were able to adhere to fibronectin and laminin immobilized on microtiter plates, and this adhesion was markedly reduced by both the anti-GAPDH PAb and soluble GAPDH from Saccharomyces cerevisiae. In addition, semiquantitative flow cytometry analysis with the anti-GAPDH PAb showed a decrease in antibody binding to cells in the presence of soluble fibronectin and laminin. Purified cytosolic C. albicans GAPDH was found to bind to fibronectin and laminin in a ligand Western blot assay. These observations suggest that the cell wall-associated form of the GAPDH in C. albicans could be involved in mediating adhesion of fungal cells to fibronectin and laminin, thus contributing to the attachment of the microorganism to host tissues and to the dissemination of Candida infection.

* Corresponding author. Mailing address: Departamento de Microbiología y Ecología, Facultad de Farmacia, Universitat de València. Avda. Vicente Andrés Estellés, s/n, 46100-Burjasot, Valencia, Spain. Phone and fax: 34-6-3864770. E-mail: M.Luisa.Gil{at}uv.es.

Infect Immun, May 1998, p. 2052-2059, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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