This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Simpson, C. L.
Right arrow Articles by Russell, R. R. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Simpson, C. L.
Right arrow Articles by Russell, R. R. B.

 Previous Article  |  Next Article 

Infect Immun, May 1998, p. 2085-2092, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of a Homolog of CcpA Catabolite Repressor Protein in Streptococcus mutans

Christine L. Simpson and Roy R. B. Russell*

Department of Oral Biology, The Dental School, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom

Received 18 August 1997/Returned for modification 17 November 1997/Accepted 3 February 1998

A locus containing a gene with homology to ccpA of other bacteria has been cloned from Streptococcus mutans LT11, sequenced, and named regM. Upstream of the regM gene, on the opposite strand, is a gene encoding an X-Pro dipeptidase, pepQ. A 14-bp palindromic sequence with homology to the consensus catabolite-responsive element sequence lay in the promoter region between the two genes. To study the function of regM, the gene was inactivated by insertion of an antibiotic resistance marker. Diauxic growth of S. mutans on a number of sugars in the presence of glucose was not affected by disruption of regM. The loss of RegM increased glucose repression of alpha -galactosidase, mannitol-1-P dehydrogenase, and P-beta -galactosidase activities. These results suggest that while RegM can affect catabolite repression in S. mutans, it does not conform to the model proposed for CcpA in Bacillus subtilis.

* Corresponding author. Mailing address: Department of Oral Biology, Dental School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4BW, United Kingdom. Phone: 44 191 222 7859. Fax: 44 191 222 6137. E-mail: r.r.russell{at}ncl.ac.uk.

Infect Immun, May 1998, p. 2085-2092, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nobbs, A. H., Lamont, R. J., Jenkinson, H. F. (2009). Streptococcus Adherence and Colonization. Microbiol. Mol. Biol. Rev. 73: 407-450 [Abstract] [Full Text]  
  • Abranches, J., Nascimento, M. M., Zeng, L., Browngardt, C. M., Wen, Z. T., Rivera, M. F., Burne, R. A. (2008). CcpA Regulates Central Metabolism and Virulence Gene Expression in Streptococcus mutans. J. Bacteriol. 190: 2340-2349 [Abstract] [Full Text]  
  • Ahn, S.-J., Wen, Z. T., Burne, R. A. (2007). Effects of Oxygen on Virulence Traits of Streptococcus mutans. J. Bacteriol. 189: 8519-8527 [Abstract] [Full Text]  
  • Kaufman, G. E., Yother, J. (2007). CcpA-Dependent and -Independent Control of Beta-Galactosidase Expression in Streptococcus pneumoniae Occurs via Regulation of an Upstream Phosphotransferase System-Encoding Operon. J. Bacteriol. 189: 5183-5192 [Abstract] [Full Text]  
  • Deutscher, J., Francke, C., Postma, P. W. (2006). How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria. Microbiol. Mol. Biol. Rev. 70: 939-1031 [Abstract] [Full Text]  
  • Waterhouse, J. C., Russell, R. R. B. (2006). Dispensable genes and foreign DNA in Streptococcus mutans. Microbiology 152: 1777-1788 [Abstract] [Full Text]  
  • Griswold, A. R., Jameson-Lee, M., Burne, R. A. (2006). Regulation and Physiologic Significance of the Agmatine Deiminase System of Streptococcus mutans UA159. J. Bacteriol. 188: 834-841 [Abstract] [Full Text]  
  • Iyer, R., Baliga, N. S., Camilli, A. (2005). Catabolite Control Protein A (CcpA) Contributes to Virulence and Regulation of Sugar Metabolism in Streptococcus pneumoniae. J. Bacteriol. 187: 8340-8349 [Abstract] [Full Text]  
  • Rathsam, C., Eaton, R. E., Simpson, C. L., Browne, G. V., Berg, T., Harty, D. W. S., Jacques, N. A. (2005). Up-regulation of competence- but not stress-responsive proteins accompanies an altered metabolic phenotype in Streptococcus mutans biofilms. Microbiology 151: 1823-1837 [Abstract] [Full Text]  
  • Asanuma, N., Yoshii, T., Hino, T. (2004). Molecular Characterization of CcpA and Involvement of This Protein in Transcriptional Regulation of Lactate Dehydrogenase and Pyruvate Formate-Lyase in the Ruminal Bacterium Streptococcus bovis. Appl. Environ. Microbiol. 70: 5244-5251 [Abstract] [Full Text]  
  • Ajdic', D., McShan, W. M., McLaughlin, R. E., Savic', G., Chang, J., Carson, M. B., Primeaux, C., Tian, R., Kenton, S., Jia, H., Lin, S., Qian, Y., Li, S., Zhu, H., Najar, F., Lai, H., White, J., Roe, B. A., Ferretti, J. J. (2002). Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proc. Natl. Acad. Sci. USA 99: 14434-14439 [Abstract] [Full Text]  
  • Giammarinaro, P., Paton, J. C. (2002). Role of RegM, a Homologue of the Catabolite Repressor Protein CcpA, in the Virulence of Streptococcus pneumoniae. Infect. Immun. 70: 5454-5461 [Abstract] [Full Text]  
  • Wen, Z. T., Burne, R. A. (2002). Functional Genomics Approach to Identifying Genes Required for Biofilm Development by Streptococcus mutans. Appl. Environ. Microbiol. 68: 1196-1203 [Abstract] [Full Text]  
  • Wen, Z. T., Burne, R. A. (2002). Analysis of cis- and trans-Acting Factors Involved in Regulation of the Streptococcus mutans Fructanase Gene (fruA). J. Bacteriol. 184: 126-133 [Abstract] [Full Text]  
  • Muscariello, L., Marasco, R., De Felice, M., Sacco, M. (2001). The Functional ccpA Gene Is Required for Carbon Catabolite Repression in Lactobacillus plantarum. Appl. Environ. Microbiol. 67: 2903-2907 [Abstract] [Full Text]  
  • Rogers, J. D., Scannapieco, F. A. (2001). RegG, a CcpA Homolog, Participates in Regulation of Amylase-Binding Protein A Gene (abpA) Expression in Streptococcus gordonii. J. Bacteriol. 183: 3521-3525 [Abstract] [Full Text]  
  • van den Bogaard, P. T. C., Kleerebezem, M., Kuipers, O. P., de Vos, W. M. (2000). Control of Lactose Transport, beta -Galactosidase Activity, and Glycolysis by CcpA in Streptococcus thermophilus: Evidence for Carbon Catabolite Repression by a Non-Phosphoenolpyruvate-Dependent Phosphotransferase System Sugar. J. Bacteriol. 182: 5982-5989 [Abstract] [Full Text]  
  • Mahr, K., Hillen, W., Titgemeyer, F. (2000). Carbon Catabolite Repression in Lactobacillus pentosus: Analysis of the ccpA Region. Appl. Environ. Microbiol. 66: 277-283 [Abstract] [Full Text]  
  • Rosenow, C., Maniar, M., Trias, J. (1999). Regulation of the alpha -Galactosidase Activity in Streptococcus pneumoniae: Characterization of the Raffinose Utilization System. Genome Res 9: 1189-1197 [Abstract] [Full Text]  
  • Plamondon, P., Brochu, D., Thomas, S., Fradette, J., Gauthier, L., Vaillancourt, K., Buckley, N., Frenette, M., Vadeboncoeur, C. (1999). Phenotypic Consequences Resulting from a Methionine-to-Valine Substitution at Position 48 in the HPr Protein of Streptococcus salivarius. J. Bacteriol. 181: 6914-6921 [Abstract] [Full Text]  
  • Schick, J., Weber, B., Klein, J. R., Henrich, B. (1999). PepR1, a CcpA-like transcription regulator of Lactobacillus delbrueckii subsp. lactis. Microbiology 145: 3147-3154 [Abstract] [Full Text]  
  • Burne, R. A., Wen, Z. T., Chen, Y.-Y. M., Penders, J. E. C. (1999). Regulation of Expression of the Fructan Hydrolase Gene of Streptococcus mutans GS-5 by Induction and Carbon Catabolite Repression. J. Bacteriol. 181: 2863-2871 [Abstract] [Full Text]  
  • Brochu, D., Vadeboncoeur, C. (1999). The HPr(Ser) Kinase of Streptococcus salivarius: Purification, Properties, and Cloning of the hprK Gene. J. Bacteriol. 181: 709-717 [Abstract] [Full Text]  
  • Behari, J., Youngman, P. (1998). A Homolog of CcpA Mediates Catabolite Control in Listeria monocytogenes but Not Carbon Source Regulation of Virulence Genes. J. Bacteriol. 180: 6316-6324 [Abstract] [Full Text]  
  • Simpson, C. L., Russell, R. R. B. (1998). Intracellular alpha -Amylase of Streptococcus mutans. J. Bacteriol. 180: 4711-4717 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»