Infect Immun, May 1998, p. 2085-2092, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Department of Oral Biology, The Dental School, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom
Received 18 August 1997/Returned for modification 17 November 1997/Accepted 3 February 1998
A locus containing a gene with homology to ccpA of
other bacteria has been cloned from Streptococcus mutans
LT11, sequenced, and named regM. Upstream of the
regM gene, on the opposite strand, is a gene encoding an
X-Pro dipeptidase, pepQ. A 14-bp palindromic sequence with
homology to the consensus catabolite-responsive element sequence lay in
the promoter region between the two genes. To study the function of
regM, the gene was inactivated by insertion of an
antibiotic resistance marker. Diauxic growth of S. mutans on a number of sugars in the presence of glucose was not affected by
disruption of regM. The loss of RegM increased glucose
repression of
-galactosidase, mannitol-1-P dehydrogenase, and
P-
-galactosidase activities. These results suggest that while RegM
can affect catabolite repression in S. mutans, it does not
conform to the model proposed for CcpA in Bacillus subtilis.
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