IAI FigSearch
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Seydel, K. B.
Right arrow Articles by Stanley, S. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Seydel, K. B.
Right arrow Articles by Stanley, S. L., Jr.

Infect Immun, May 1998, p. 2379-2382, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cryptosporidium parvum Infection of Human Intestinal Xenografts in SCID Mice Induces Production of Human Tumor Necrosis Factor Alpha and Interleukin-8

Karl B. Seydel,1 Tonghai Zhang,2 Gretchen A. Champion,2 Carl Fichtenbaum,2 Paul E. Swanson,3 Saul Tzipori,4 Jeffrey K. Griffiths,4,5 and Samuel L. Stanley Jr.1,2,*

Departments of Molecular Microbiology,1 Medicine,2 and Pathology,3 Washington University School of Medicine, St. Louis, Missouri, and Division of Infectious Diseases, Department of Biomedical Sciences, Tufts University School of Veterinary Medicine,4 and Department of Family Health and Community Medicine, Tufts University School of Medicine,5 Boston, Massachusetts

Received 17 November 1997/Returned for modification 14 January 1998/Accepted 26 January 1998

The protozoan parasite Cryptosporidium parvum invades intestinal epithelial cells and can cause life-threatening diarrhea in immunocompromised individuals. Despite the clinical importance of this organism, much remains to be learned about the pathogenesis of C. parvum-induced diarrhea. To explore the role of the intestinal inflammatory response in C. parvum disease, using C. parvum oocysts we infected human intestinal xenografts in severe combined immunodeficient (SCID) mice. Seven days after infection, we found levels of human tumor necrosis factor alpha and interleukin-8 in C. parvum-infected human intestinal xenografts that were significantly higher than those seen in uninfected control xenografts. These results demonstrate that human intestinal cells produce proinflammatory cytokines in response to C. parvum infection and establish SCID-HU-INT mice as a model system to study the interactions of C. parvum with the human intestine.

* Corresponding author. Mailing address: Washington University School of Medicine, 660 S. Euclid Ave., Box 8051, St. Louis, MO 63110. Phone: (314) 362-1070. Fax: (314) 362-3525. E-mail: sstanley{at}im.wustl.edu.

Infect Immun, May 1998, p. 2379-2382, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 1998 by the American Society for Microbiology. All rights reserved.