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Infect Immun, May 1998, p. 2383-2386, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Control of Ferredoxin and Gal/GalNAc Lectin Gene Expression in Entamoeba histolytica by a cis-Acting DNA Sequence

Carol A. Gilchrist,1 Barbara J. Mann,1,2 and William A. Petri Jr.1,2,3,*

Departments of Internal Medicine,1 Microbiology,2 and Pathology,3 University of Virginia, Charlottesville, Virginia 22908

Received 20 November 1997/Returned for modification 6 January 1998/Accepted 26 January 1998

The ferredoxin (fdx) and lectin (hgl5) promoters of Entamoeba histolytica contain the DNA sequence motif TATTCTATT (URE3). Previously we showed that mutation of the URE3 motif in the hgl5 lectin promoter results in an increase in promoter reporter activity. Mutation of this motif in the fdx promoter led to a 40-to-50% decrease in fdx promoter activity as measured by reporter gene activity and abundance of mRNA. E. histolytica nuclear proteins exhibited sequence-specific binding to the URE3 motif in electrophoretic mobility shift assays. These results support the regulation of both ferredoxin and lectin promoters by a nuclear protein(s) which recognizes the URE3 motif.

* Corresponding author. Mailing address: Rm. 2115, MR4 Bldg., University of Virginia Health Sciences Center, Charlottesville, VA 22908. Phone: (804) 924-5621. Fax: (804) 924-0075. E-mail: wap3g{at}virginia.edu.

Infect Immun, May 1998, p. 2383-2386, Vol. 66, No. 5
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hackney, J. A., Ehrenkaufer, G. M., Singh, U. (2007). Identification of putative transcriptional regulatory networks in Entamoeba histolytica using Bayesian inference. Nucleic Acids Res 35: 2141-2152 [Abstract] [Full Text]  
  • Gilchrist, C. A., Leo, M., Line, C. G., Mann, B. J., Petri, W. A. Jr. (2003). Calcium Modulates Promoter Occupancy by the Entamoeba histolytica Ca2+-binding Transcription Factor URE3-BP. J. Biol. Chem. 278: 4646-4653 [Abstract] [Full Text]  
  • Singh, U., Rogers, J. B. (1998). The Novel Core Promoter Element GAAC in the hgl5 Gene of Entamoeba histolytica Is Able to Direct a Transcription Start Site Independent of TATA or Initiator Regions. J. Biol. Chem. 273: 21663-21668 [Abstract] [Full Text]  
  • Schaenman, J. M., Gilchrist, C. A., Mann, B. J., Petri, W. A. Jr. (2001). Identification of Two Entamoeba histolytica Sequence-specific URE4 Enhancer-binding Proteins with Homology to the RNA-binding Motif RRM. J. Biol. Chem. 276: 1602-1609 [Abstract] [Full Text]  
  • Gilchrist, C. A., Holm, C. F., Hughes, M. A., Schaenman, J. M., Mann, B. J., Petri, W. A. Jr. (2001). Identification and Characterization of an Entamoeba histolytica Upstream Regulatory Element 3 Sequence-specific DNA-binding Protein Containing EF-hand Motifs. J. Biol. Chem. 276: 11838-11843 [Abstract] [Full Text]  


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