This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hopkins, W. J.
Right arrow Articles by Uehling, D. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hopkins, W. J.
Right arrow Articles by Uehling, D. T.

 Previous Article  |  Next Article 

Infect Immun, June 1998, p. 2798-2802, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Time Course and Host Responses to Escherichia coli Urinary Tract Infection in Genetically Distinct Mouse Strains

Walter J. Hopkins,1,* Annette Gendron-Fitzpatrick,2 Edward Balish,1 and David T. Uehling1

Department of Surgery1 and Research Animal Resource Center,2 University of Wisconsin, Madison, Wisconsin

Received 28 July 1997/Returned for modification 1 September 1997/Accepted 23 March 1998

Recurrent urinary tract infections (UTIs) are a significant clinical problem for many women; however, host susceptibility factors have not been completely defined. The mouse model of induced UTI provides an experimental environment in which to identify specific host characteristics that are important in initial bacterial colonization of the urinary tract and in resolution of an infection. This study examined initial susceptibility, bacterial clearance, and host defense mechanisms during induction and resolution of Escherichia coli UTIs in genetically distinct strains of mice. Of the ten inbred strains tested, six (BALB/c, C3H/HeN, C57BL/6, DBA.1, DBA.2, and AKR) showed progressive resolution of bladder infections over a 14-day period. A constant, low-level bladder infection was observed in SWR and SJL mice. High bladder infection levels persisted over the 14-day study period in C3H/HeJ and C3H/OuJ mice. Kidney infection levels generally correlated with bladder infection levels, especially in C3H/HeJ and C3H/OuJ mice, the two most susceptible strains, in which infections became more severe with time after challenge. The degree of inflammation in bladder and kidneys, as well as antibody-forming cell responses, positively correlated with infection intensity in all strains except C3H/HeJ, which had minimal inflammation despite high infection levels. These results demonstrate two important aspects of host defense against UTI. First, the innate immune response to an infection in the bladder or kidneys consists primarily of local inflammation, which is followed by an adaptive response characterized in part by an antibody response to the infecting bacteria. Second, a UTI will be spontaneously resolved in most cases; however, in mice with specific genetic backgrounds, a UTI can persist for an extended length of time. The latter result strongly suggests that the presence or absence of specific host genes will determine how effectively an E. coli UTI will be resolved.

* Corresponding author. Mailing address: Department of Surgery, 600 Highland Ave., Madison, WI 53792. Phone: (608) 263-0887. Fax: (608) 263-0454. E-mail: hopkins{at}surgery.wisc.edu.

Infect Immun, June 1998, p. 2798-2802, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Roelofs, J. J. T. H., Rouschop, K. M. A., Teske, G. J. D., Wagenaar, G. T. M., Claessen, N., Weening, J. J., van der Poll, T., Florquin, S. (2009). Endogenous tissue-type plasminogen activator is protective during ascending urinary tract infection. Nephrol Dial Transplant 24: 801-808 [Abstract] [Full Text]  
  • Andersen-Nissen, E., Hawn, T. R., Smith, K. D., Nachman, A., Lampano, A. E., Uematsu, S., Akira, S., Aderem, A. (2007). Cutting Edge: Tlr5-/- Mice Are More Susceptible to Escherichia coli Urinary Tract Infection. J. Immunol. 178: 4717-4720 [Abstract] [Full Text]  
  • Garofalo, C. K., Hooton, T. M., Martin, S. M., Stamm, W. E., Palermo, J. J., Gordon, J. I., Hultgren, S. J. (2007). Escherichia coli from Urine of Female Patients with Urinary Tract Infections Is Competent for Intracellular Bacterial Community Formation. Infect. Immun. 75: 52-60 [Abstract] [Full Text]  
  • Casaz, P., Garrity-Ryan, L. K., McKenney, D., Jackson, C., Levy, S. B., Tanaka, S. K., Alekshun, M. N. (2006). MarA, SoxS and Rob function as virulence factors in an Escherichia coli murine model of ascending pyelonephritis. Microbiology 152: 3643-3650 [Abstract] [Full Text]  
  • Rouschop, K. M. A., Sylva, M., Teske, G. J. D., Hoedemaeker, I., Pals, S. T., Weening, J. J., van der Poll, T., Florquin, S. (2006). Urothelial CD44 Facilitates Escherichia coli Infection of the Murine Urinary Tract. J. Immunol. 177: 7225-7232 [Abstract] [Full Text]  
  • Yohe, H. C., O'Hara, K. A., Hunt, J. A., Kitzmiller, T. J., Wood, S. G., Bement, J. L., Bement, W. J., Szakacs, J. G., Wrighton, S. A., Jacobs, J. M., Kostrubsky, V., Sinclair, P. R., Sinclair, J. F. (2006). Involvement of Toll-like receptor 4 in acetaminophen hepatotoxicity. Am. J. Physiol. Gastrointest. Liver Physiol. 290: G1269-G1279 [Abstract] [Full Text]  
  • Thumbikat, P., Waltenbaugh, C., Schaeffer, A. J., Klumpp, D. J. (2006). Antigen-specific responses accelerate bacterial clearance in the bladder.. J. Immunol. 176: 3080-3086 [Abstract] [Full Text]  
  • Schilling, J. D., Martin, S. M., Hung, C. S., Lorenz, R. G., Hultgren, S. J. (2003). Toll-like receptor 4 on stromal and hematopoietic cells mediates innate resistance to uropathogenic Escherichiacoli. Proc. Natl. Acad. Sci. USA 100: 4203-4208 [Abstract] [Full Text]  
  • Schilling, J. D., Martin, S. M., Hunstad, D. A., Patel, K. P., Mulvey, M. A., Justice, S. S., Lorenz, R. G., Hultgren, S. J. (2003). CD14- and Toll-Like Receptor-Dependent Activation of Bladder Epithelial Cells by Lipopolysaccharide and Type 1 Piliated Escherichia coli. Infect. Immun. 71: 1470-1480 [Abstract] [Full Text]  
  • Poljakovic, M., Persson, K. (2003). Urinary tract infection in iNOS-deficient mice with focus on bacterial sensitivity to nitric oxide. Am. J. Physiol. Renal Physiol. 284: F22-F31 [Abstract] [Full Text]  
  • Zhou, G., Mo, W.-J., Sebbel, P., Min, G., Neubert, T. A., Glockshuber, R., Wu, X.-R., Sun, T.-T., Kong, X.-P. (2002). Uroplakin Ia is the urothelial receptor for uropathogenic Escherichia coli: evidence from in vitro FimH binding. J. Cell Sci. 114: 4095-4103 [Abstract] [Full Text]  
  • Mulvey, M. A., Schilling, J. D., Hultgren, S. J. (2001). Establishment of a Persistent Escherichia coli Reservoir during the Acute Phase of a Bladder Infection. Infect. Immun. 69: 4572-4579 [Abstract] [Full Text]  
  • Asahara, T., Nomoto, K., Watanuki, M., Yokokura, T. (2001). Antimicrobial Activity of Intraurethrally Administered Probiotic Lactobacillus casei in a Murine Model of Escherichia coli Urinary Tract Infection. Antimicrob. Agents Chemother. 45: 1751-1760 [Abstract] [Full Text]  
  • Rippere-Lampe, K. E., O'Brien, A. D., Conran, R., Lockman, H. A. (2001). Mutation of the Gene Encoding Cytotoxic Necrotizing Factor Type 1 (cnf1) Attenuates the Virulence of Uropathogenic Escherichia coli. Infect. Immun. 69: 3954-3964 [Abstract] [Full Text]  
  • Means, T. K., Jones, B. W., Schromm, A. B., Shurtleff, B. A., Smith, J. A., Keane, J., Golenbock, D. T., Vogel, S. N., Fenton, M. J. (2001). Differential Effects of a Toll-Like Receptor Antagonist on Mycobacterium tuberculosis-Induced Macrophage Responses. J. Immunol. 166: 4074-4082 [Abstract] [Full Text]  
  • Schilling, J. D., Mulvey, M. A., Vincent, C. D., Lorenz, R. G., Hultgren, S. J. (2001). Bacterial Invasion Augments Epithelial Cytokine Responses to Escherichia coli Through a Lipopolysaccharide-Dependent Mechanism. J. Immunol. 166: 1148-1155 [Abstract] [Full Text]  
  • Mulvey, M. A., Schilling, J. D., Martinez, J. J., Hultgren, S. J. (2000). From the Cover: Bad bugs and beleaguered bladders: Interplay between uropathogenic Escherichia coli and innate host defenses. Proc. Natl. Acad. Sci. USA 97: 8829-8835 [Abstract] [Full Text]  
  • Johnson;, J. R., Hopkins, W. J. (1998). Reflux in the Mouse Model of Urinary Tract Infection. Infect. Immun. 66: 6063-6064 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»