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Infect Immun, June 1998, p. 2827-2835, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Humoral Immunity to Borrelia burgdorferi
N40 Decorin Binding Proteins during Infection of Laboratory
Mice
Sunlian
Feng,1
Emir
Hodzic,1
Brian
Stevenson,2 and
Stephen W.
Barthold1,*
Center for Comparative Medicine, Schools of
Medicine and Veterinary Medicine, University of California, Davis,
California 95616,1 and
Laboratory of
Microbial Structure and Function, Rocky Mountain
Laboratories, National Institute of Allergy and Infectious Diseases,
Hamilton, Montana 598402
Received 9 September 1997/Returned for modification 19 November
1997/Accepted 6 March 1998
A Borrelia burgdorferi N40 genomic expression library
was screened with serum from actively infected mice to identify gene products that elicit protective immunity. A clone that contained a
putative bicistronic operon containing two genes that encoded 20- and
22-kDa lipoproteins was identified and sequenced. These genes showed
homology with the genes encoding decorin binding proteins DbpB and
DbpA, respectively, of B. burgdorferi 297 and B31.
N40-dbpA DNA hybridized with B. burgdorferi N40
DNA on a single 48-kb linear plasmid. Homologous genes could be
amplified under various degrees of stringency by PCR or hybridized by
Southern blotting from B. burgdorferi sensu stricto N40 and
B31, and from B. burgdorferi sensu lato PBi and 25015, but
not PKo. Recombinant N40-DbpB and N40-DbpA were reactive with antibody
in serum from infected mice, and serum was more reactive against
N40-DbpA than against B. burgdorferi N40 recombinant P39,
OspC, or OspA. Sera from mice infected with B. burgdorferi
sensu lato strains PKo and PBi were weakly reactive against N40-DbpB
and N40-DbpA, and sera from mice infected with 25015 were moderately
reactive, compared to sera from mice infected with B. burgdorferi N40. Hyperimmunization of mice with N40-DbpA, but not
N40-DbpB, induced protective immunity against syringe challenge with
cultured B. burgdorferi N40. DbpA may therefore be one of
the antigens responsible for eliciting protective antibody known to
exist in serum from infected mice. DNA amplification and serology
suggest that DbpB and DbpA are likely to have homologs throughout the
B. burgdorferi sensu lato family, but they are likely to be
heterogeneous.
*
Corresponding author. Mailing address: Center for
Comparative Medicine, University of California, One Shields Avenue,
Davis, CA 95616. Phone: (530) 752-7913. Fax: (530) 752-7914. E-mail: swbarthold{at}ucdavis.edu.
Infect Immun, June 1998, p. 2827-2835, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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