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Infect Immun, June 1998, p. 2887-2894, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of the Type 3 Fimbrial Adhesins of Klebsiella Strains

Tricia A. Schurtz Sebghati,1 Timo K. Korhonen,2 Douglas B. Hornick,3 and Steven Clegg1,*

Departments of Microbiology1 and Internal Medicine,3 University of Iowa College of Medicine, Iowa City, Iowa 52242, and Department of General Microbiology, University of Helsinki, Helsinki, Finland2

Received 5 December 1997/Returned for modification 10 February 1998/Accepted 27 March 1998

The Klebsiella pneumoniae fimbrial adhesin, MrkD, mediates adherence to the basolateral surfaces of renal and pulmonary epithelia and to the basement membranes of tissues. Although all isolates possessing the MrkD adhesin mediate the agglutination, in vitro, of erythrocytes treated with tannic acid, the mrkD gene is not conserved within species. The ability of a plasmid-borne mrkD gene product to mediate binding to type V collagen is associated frequently with strains of K. oxytoca and rarely with strains of K. pneumoniae. In K. pneumoniae, the MrkD adhesin is located within a chromosomally borne gene cluster and mediates binding to collagen types IV and V. The plasmid-borne determinant, mrkD1P, and the chromosomally borne gene, mrkD1C, are not genetically related. Some strains of enterobacteria possess a mrkD1C allele that is associated with hemagglutinating activity but does not bind to either type IV or type V collagen.

* Corresponding author. Mailing address: Department of Microbiology, 3403 Bowen Science Building, University of Iowa College of Medicine, Iowa City, IA 52242-1109. Phone: (319) 335-7778. Fax: (319) 335-9006. E-mail: steven-clegg{at}uiowa.edu.

Infect Immun, June 1998, p. 2887-2894, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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