Infect Immun, June 1998, p. 2938-2942, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Medical Service,
Received 1 October 1997/Returned for modification 8 January
1998/Accepted 13 March 1998
We have developed an animal model for studying mycobacterial
pathogenesis using Mycobacterium marinum and the goldfish,
Carassius auratus. Goldfish are injected intraperitoneally
with doses between 102 and 109 CFU of M. marinum organisms. Depending on the dose of M. marinum organisms administered, an acute or chronic disease is
produced. The acute disease is characterized by systemic mycobacterial
infection, severe peritonitis, tissue necrosis, and a short median
survival time. The chronic disease is characterized by granuloma
formation in all organs and survival of animals to the end point of the experiment (56 days). Colony counts in organ homogenates showed recovery of mycobacteria from a high percentage of inoculated animals.
We believe this well-characterized animal model will be useful for
studying mycobacterial pathogenesis.
*
Corresponding author. Mailing address: Center for
Vaccine Development, Division of Geographic Medicine, University of
Maryland School of Medicine, 685 W. Baltimore St., Baltimore, MD 21201. Phone: (410) 706-5328. Fax: (410) 706-6205. E-mail:
mtrucksi{at}umppa1.ab.umd.edu.
This article has been cited by other articles:
| J. Bacteriol. | J. Virol. | Eukaryot. Cell |
|---|
| Microbiol. Mol. Biol. Rev. | Clin. Vaccine Immunol. | All ASM Journals |
|---|