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Infect Immun, June 1998, p. 2951-2959, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evaluation of New Vaccines in the Mouse and Guinea Pig Model of Tuberculosis

Susan L. Baldwin,1 Celine D'Souza,1 Alan D. Roberts,1 Brian P. Kelly,1 Anthony A. Frank,2 Margaret A. Lui,3 Jeffrey B. Ulmer,3 Kris Huygen,4 David M. McMurray,5 and Ian M. Orme1,*

Mycobacteria Research Laboratories, Department of Microbiology,1 and Department of Pathology,2 Colorado State University, Fort Collins, Colorado 80523; Department of Virus and Cell Biology, Merck Research Laboratories, West Point, Pennsylvania 194863; Department of Virology, Pasteur Institute, B-1180 Brussels, Belgium4; and Department of Medical Microbiology and Immunology, Texas A&M University, College Station, Texas 778435

Received 20 October 1997/Returned for modification 8 January 1998/Accepted 12 March 1998

The results of this study provide the first evidence that two completely separate vaccine approaches, one based on a subunit vaccine consisting of a mild adjuvant admixed with purified culture filtrate proteins and enhanced by the cytokine interleukin-2 and the second based on immunization with DNA encoding the Ag85A protein secreted by Mycobacterium tuberculosis, could both prevent the onset of caseating disease, which is the hallmark of the guinea pig aerogenic infection model. In both cases, however, the survival of vaccinated guinea pigs was shorter than that conferred by Mycobacterium bovis BCG, with observed mortality of these animals probably due to consolidation of lung tissues by lymphocytic granulomas. An additional characteristic of these approaches was that neither induced skin test reactivity to commercial tuberculin. These data thus provide optimism that development of nonliving vaccines which can generate long-lived immunity approaching that conferred by the BCG vaccine is a feasible goal.

* Corresponding author. Mailing address: Mycobacteria Research Laboratories, Department of Microbiology, Colorado State University, Fort Collins, CO 80523. Phone: (970) 491-5777. Fax: (970) 491-5125. E-mail: iorme{at}lamar.colostate.edu.

Infect Immun, June 1998, p. 2951-2959, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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