This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An author's correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fernandez, V.
Right arrow Articles by Wahlgren, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fernandez, V.
Right arrow Articles by Wahlgren, M.

 Previous Article  |  Next Article 

Infect Immun, June 1998, p. 2969-2975, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Multiple Adhesive Phenotypes Linked to Rosetting Binding of Erythrocytes in Plasmodium falciparum Malaria

Victor Fernandez,1 Carl Johan Treutiger,1 Gerard B. Nash,2 and Mats Wahlgren1,*

Microbiology and Tumor Biology Center, Karolinska Institutet, and Swedish Institute for Infectious Disease Control, S-171 77 Stockholm, Sweden,1 and Department of Physiology, University of Birmingham, Birmingham B15 2TT, United Kingdom2

Received 8 December 1997/Returned for modification 5 February 1998/Accepted 13 March 1998

The cerebral form of severe malaria is associated with excessive intravascular sequestration of Plasmodium falciparum-infected erythrocytes (PRBC). Retention and accumulation of PRBC may lead to occlusion of brain microvessels and direct the triggering of acute pathologic changes. Here we report that by selection, cloning, and subcloning, we have identified rare P. falciparum parasites expressing a pan-adhesive phenotype linked to erythrocyte rosetting, a previously identified correlate of cerebral malaria. Rosetting PRBC not only bound uninfected erythrocytes but also formed autoagglutinates, adhered to endothelial cells, and bound to CD36, immunoglobulins, and the blood group A antigen. The linkage of rosetting, autoagglutination, and cytoadherence involved the coexpression on a single PRBC of ligands with multiple specificities and the binding to two or more receptors on erythrocytes and to at least two other cell adhesion molecules, including a new endothelial cell receptor for P. falciparum-infected erythrocytes. Limited proteolysis that differentially cleaved the rosetting ligand PfEMP1 from the PRBC surface abrogated all the binding phenotypes of these parasites, implicating the variant antigen PfEMP1 as a carrier of multiple ligand specificities. The results encourage the further study of pan-adhesion as a potentially important parasite phenotype in the pathogenesis of severe P. falciparum malaria.

* Corresponding author. Mailing address: MTC, Karolinska Institute, Box 280, 171 77 Stockholm, Sweden. Phone: 46 8 7287277. Fax: 46 8 331547. E-mail: mats.wahlgren{at}smi.ki.se.

Infect Immun, June 1998, p. 2969-2975, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Karuppiah, S., Rodgman, C., Lombard, J. (2009). Dyke-Davidoff-Masson Syndrome in Postcerebral Malaria. J Child Neurol 24: 487-490 [Abstract]  
  • Raj, D. K., Mu, J., Jiang, H., Kabat, J., Singh, S., Sullivan, M., Fay, M. P., McCutchan, T. F., Su, X.-z. (2009). Disruption of a Plasmodium falciparum Multidrug Resistance-associated Protein (PfMRP) Alters Its Fitness and Transport of Antimalarial Drugs and Glutathione. J. Biol. Chem. 284: 7687-7696 [Abstract] [Full Text]  
  • Vigan-Womas, I., Guillotte, M., Le Scanf, C., Igonet, S., Petres, S., Juillerat, A., Badaut, C., Nato, F., Schneider, A., Lavergne, A., Contamin, H., Tall, A., Baril, L., Bentley, G. A., Mercereau-Puijalon, O. (2008). An In Vivo and In Vitro Model of Plasmodium falciparum Rosetting and Autoagglutination Mediated by varO, a Group A var Gene Encoding a Frequent Serotype. Infect. Immun. 76: 5565-5580 [Abstract] [Full Text]  
  • Cserti, C. M., Dzik, W. H. (2007). The ABO blood group system and Plasmodium falciparum malaria. Blood 110: 2250-2258 [Abstract] [Full Text]  
  • Luginbuhl, A., Nikolic, M., Beck, H. P., Wahlgren, M., Lutz, H. U. (2007). Complement Factor D, Albumin, and Immunoglobulin G Anti-Band 3 Protein Antibodies Mimic Serum in Promoting Rosetting of Malaria-Infected Red Blood Cells. Infect. Immun. 75: 1771-1777 [Abstract] [Full Text]  
  • HAEGGSTROM, M., VON EULER, A., KIRONDE, F., FERNANDEZ, V., WAHLGREN, M. (2007). OF MAURER'S CLEFTS IN PLASMODIUM FALCIPARUM-INFECTED ERYTHROCYTES. Am J Trop Med Hyg 76: 27-32 [Abstract] [Full Text]  
  • Moll, K., Pettersson, F., Vogt, A. M., Jonsson, C., Rasti, N., Ahuja, S., Spangberg, M., Mercereau-Puijalon, O., Arnot, D. E., Wahlgren, M., Chen, Q. (2007). Generation of Cross-Protective Antibodies against Plasmodium falciparum Sequestration by Immunization with an Erythrocyte Membrane Protein 1-Duffy Binding-Like 1{alpha} Domain. Infect. Immun. 75: 211-219 [Abstract] [Full Text]  
  • Pettersson, F., Vogt, A. M., Jonsson, C., Mok, B. W., Shamaei-Tousi, A., Bergstrom, S., Chen, Q., Wahlgren, M. (2005). Whole-Body Imaging of Sequestration of Plasmodium falciparum in the Rat. Infect. Immun. 73: 7736-7746 [Abstract] [Full Text]  
  • Donati, D., Zhang, L. P., Chen, Q., Chene, A., Flick, K., Nystrom, M., Wahlgren, M., Bejarano, M. T. (2004). Identification of a Polyclonal B-Cell Activator in Plasmodium falciparum. Infect. Immun. 72: 5412-5418 [Abstract] [Full Text]  
  • O'Dea, K. P., Pasvol, G. (2003). Optimal Tumor Necrosis Factor Induction by Plasmodium falciparum Requires the Highly Localized Release of Parasite Products. Infect. Immun. 71: 3155-3164 [Abstract] [Full Text]  
  • Miller, S. K., Good, R. T., Drew, D. R., Delorenzi, M., Sanders, P. R., Hodder, A. N., Speed, T. P., Cowman, A. F., de Koning-Ward, T. F., Crabb, B. S. (2002). A Subset of Plasmodium falciparum SERA Genes Are Expressed and Appear to Play an Important Role in the Erythrocytic Cycle. J. Biol. Chem. 277: 47524-47532 [Abstract] [Full Text]  
  • Lou, J., Lucas, R., Grau, G. E. (2001). Pathogenesis of Cerebral Malaria: Recent Experimental Data and Possible Applications for Humans. Clin. Microbiol. Rev. 14: 810-820 [Abstract] [Full Text]  
  • Heddini, A., Pettersson, F., Kai, O., Shafi, J., Obiero, J., Chen, Q., Barragan, A., Wahlgren, M., Marsh, K. (2001). Fresh Isolates from Children with Severe Plasmodium falciparum Malaria Bind to Multiple Receptors. Infect. Immun. 69: 5849-5856 [Abstract] [Full Text]  
  • Pain, A., Ferguson, D. J. P., Kai, O., Urban, B. C., Lowe, B., Marsh, K., Roberts, D. J. (2001). Platelet-mediated clumping of Plasmodium falciparum-infected erythrocytes is a common adhesive phenotype and is associated with severe malaria. Proc. Natl. Acad. Sci. USA 98: 1805-1810 [Abstract] [Full Text]  
  • Hearn, J., Rayment, N., Landon, D. N., Katz, D. R., de Souza, J. B. (2000). Immunopathology of Cerebral Malaria: Morphological Evidence of Parasite Sequestration in Murine Brain Microvasculature. Infect. Immun. 68: 5364-5376 [Abstract] [Full Text]  
  • Chen, Q., Schlichtherle, M., Wahlgren, M. (2000). Molecular Aspects of Severe Malaria. Clin. Microbiol. Rev. 13: 439-450 [Abstract] [Full Text]  
  • Chen, Q., Heddini, A., Barragan, A., Fernandez, V., Pearce, S. F. A., Wahlgren, M. (2000). The Semiconserved Head Structure of Plasmodium falciparum Erythrocyte Membrane Protein 1 Mediates Binding to Multiple Independent Host Receptors. JEM 192: 1-10 [Abstract] [Full Text]  
  • Barragan, A., Fernandez, V., Chen, Q., von Euler, A., Wahlgren, M., Spillmann, D. (2000). The Duffy-binding-like domain 1 of Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is a heparan sulfate ligand that requires 12 mers for binding. Blood 95: 3594-3599 [Abstract] [Full Text]  
  • Barragan, A., Kremsner, P. G., Wahlgren, M., Carlson, J. (2000). Blood Group A Antigen Is a Coreceptor in Plasmodium falciparum Rosetting. Infect. Immun. 68: 2971-2975 [Abstract] [Full Text]  
  • Fernandez, V., Hommel, M., Chen, Q., Hagblom, P., Wahlgren, M. (1999). Small, Clonally Variant Antigens Expressed on the Surface of the Plasmodium falciparum-infected Erythrocyte Are Encoded by the rif Gene Family and Are the Target of Human Immune Responses. JEM 190: 1393-1404 [Abstract] [Full Text]  
  • Kyes, S. A., Rowe, J. A., Kriek, N., Newbold, C. I. (1999). Rifins: A second family of clonally variant proteins expressed on the surface of red cells infected with Plasmodium falciparum. Proc. Natl. Acad. Sci. USA 96: 9333-9338 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»