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Infect Immun, June 1998, p. 2987-2990, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Formation of Ring-Shaped Structures on Erythrocyte Membranes after Treatment with Botulinolysin, a Thiol-Activated Hemolysin from Clostridium botulinum

Kachiko Sekiya,1,* Hirofumi Danbara,1 Yutaka Futaesaku,2 Abdul Haque,3 Nakaba Sugimoto,3 and Morihiro Matsuda4

Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641,1 Department of Histology and Analytical Morphology, School of Allied Health Sciences, Kitasato University, Sagamihara, Kanagawa 228-8555,2 Department of Bacterial Toxinology, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Osaka 565-0871,3 and College of Nutrition, Koshien University, 10-1 Momijigaoka, Takarazuka, Hyogo 665-0006,4 Japan

Received 2 December 1997/Returned for modification 12 January 1998/Accepted 5 March 1998

Damage to erythrocyte membranes by botulinolysin (BLY) was studied by electron microscopy, which revealed ring-shaped structures with inner diameters and widths of approximately 32 and 6.7 nm, respectively. BLY bound to membranes at 0°C, but subsequent treatment with glutaraldehyde prevented ring formation during further incubation at 37°C. Zn2+ ions inhibited ring formation but not binding of BLY to membranes.

* Corresponding author. Mailing address: Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan. Phone: 81-3444-6161. Fax: 81-3444-4831. E-mail: sekiyak{at}platinum.pharm.kitasato-u.ac.jp.

Infect Immun, June 1998, p. 2987-2990, Vol. 66, No. 6
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.




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