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Infect Immun, July 1998, p. 3059-3065, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of Escherichia coli Strains Recovered from Human Cystitis and Pyelonephritis Infections in Transurethrally Challenged Mice

David E. Johnson,1,2,* C. Virginia Lockatell,1 Robert G. Russell,3,4 J. Richard Hebel,5 Michael D. Island,1 Ann Stapleton,6 Walter E. Stamm,6 and John W. Warren1

Division of Infectious Diseases, Department of Medicine,1 Program of Comparative Medicine,3 Department of Pathology,4 and Department of Epidemiology,5 University of Maryland School of Medicine, and Research Service, Department of Veterans Affairs,2 Baltimore, Maryland 21201, and Department of Medicine, University of Washington School of Medicine, Seattle, Washington 981956

Received 15 January 1998/Returned for modification 20 February 1998/Accepted 1 April 1998

Urinary tract infection, most frequently caused by Escherichia coli, is one of the most common bacterial infections in humans. A vast amount of literature regarding the mechanisms through which E. coli induces pyelonephritis has accumulated. Although cystitis accounts for 95% of visits to physicians for symptoms of urinary tract infections, few in vivo studies have investigated possible differences between E. coli recovered from patients with clinical symptoms of cystitis and that from patients with symptoms of pyelonephritis. Epidemiological studies indicate that cystitis-associated strains appear to differ from pyelonephritis-associated strains in elaboration of some putative virulence factors. With transurethrally challenged mice we studied possible differences using three each of the most virulent pyelonephritis and cystitis E. coli strains in our collection. The results indicate that cystitis strains colonize the bladder more rapidly than do pyelonephritis strains, while the rates of kidney colonization are similar. Cystitis strains colonize the bladder in higher numbers, induce more pronounced histologic changes in the bladder, and are more rapidly eliminated from the mouse urinary tract than pyelonephritis strains. These results provide evidence that cystitis strains differ from pyelonephritis strains in this model, that this model is useful for the study of the uropathogenicity of cystitis strains, and that it would be unwise to use pyelonephritis strains to study putative virulence factors important in the development of cystitis.

* Corresponding author. Mailing address: Research Service (151), VA Medical Center, 10 North Greene St., Baltimore, MD 21201. Phone: (410) 605-7130. Fax: (410) 605-7906. E-mail: dejohnso{at}umaryland.edu.

Infect Immun, July 1998, p. 3059-3065, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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