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Infect Immun, July 1998, p. 3066-3071, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Effect of Mild Acid Treatment on the Survival, Enteropathogenicity, and Protein Production in Vibrio parahaemolyticus

Hin-chung Wong,1,* Po-Yen Peng,2 Jun-Ming Han,1 Chia-Yu Chang,1 and Shang-Lun Lan1

Department of Microbiology, Soochow University, Taipei, Taiwan 111,1 and Research Section, Hsin-Chu Blood Center, Chinese Blood Service Foundation, Hsin-Chu, Taiwan 300,2 Republic of China

Received 20 October 1997/Returned for modification 11 December 1997/Accepted 8 April 1998

Vibrio parahaemolyticus is an important food-borne enteropathogen that encounters various adverse conditions in its native environment or during infection. Effects of mild acid treatment on survival under stress conditions, enteropathogenicity, and protein production in this pathogen were investigated. Logarithmically grown cells, at pH 7.5 shifted to pH 5.0 for 30 min, were more resistant to subsequent acid challenge at pH 4.4. A two-phase adaptive procedure (pH 5.8 for 30 min; pH 5.0 for 30 min) was better than a single-phase procedure for enhancing the acid tolerance of this pathogen. The acid-adapted cells were cross-protected against the challenges of low salinity and thermal inactivation. One-dimensional polyacrylamide gel electrophoresis revealed that proteins with molecular masses of 6.4, 9.0, 13.6, 16.3, 18.9, 22.9, 24.4, 28.3, 33.9, 36.9, 41.2, 47.6, 58.1, 65.6, 80.5, 88.2, and 96.9 kDa were induced or significantly enhanced, while proteins of 25.3, 30.1, 30.7, and 91.7 kDa were significantly inhibited. Two-dimensional polyacrylamide gel electrophoresis revealed that 20 species of proteins were induced or significantly enhanced, while 26 species were inhibited. In assays conducted using the suckling mouse model, enteropathogenicity of the acid-adapted cells was significantly enhanced in terms of intestine/body weight ratio and in vivo recovery of infected cells.

* Corresponding author. Mailing address: Department of Microbiology, Soochow University, Taipei, Taiwan 111, Republic of China. Phone: (886) 02-28819471, ext. 6852. Fax: (886) 02-28831193. E-mail: wonghc{at}mbm1.scu.edu.tw.

Infect Immun, July 1998, p. 3066-3071, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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