This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gillaspy, A. F.
Right arrow Articles by Smeltzer, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gillaspy, A. F.
Right arrow Articles by Smeltzer, M. S.

 Previous Article  |  Next Article 

Infect Immun, July 1998, p. 3170-3178, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Factors Affecting the Collagen Binding Capacity of Staphylococcus aureus

Allison F. Gillaspy,1 Chia Y. Lee,2 Subrata Sau,2 Ambrose L. Cheung,3 and Mark S. Smeltzer1,*

Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205;1 Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 661602; and Laboratory of Bacteriology and Immunology, Rockefeller University, New York, New York 100213

Received 2 October 1997/Returned for modification 14 January 1998/Accepted 9 February 1998

To determine whether the ability of Staphylococcus aureus to bind collagen involves an adhesin other than the collagen adhesin encoded by cna, we examined the collagen binding capacity (CBC) of 32 strains of S. aureus. With only two exceptions, a high CBC corresponded with the presence of cna. Both exceptions involved cna-positive strains with a low CBC. The first was a single strain (ACH5) that encoded but did not express cna. The second were the mucoid strains Smith diffuse and M, both of which encoded and expressed cna but bound only minimal amounts of collagen. Analysis of capsule mutants suggests that the reduced CBC observed in the mucoid strains was due to masking of the collagen adhesin on the cell surface and that this masking effect is restricted to heavily encapsulated strains. Differences in the CBC of the remaining cna-positive strains were correlated to variations in the level of cna transcription and were independent of the number of B domain repeats in the cna gene. In all cna-positive strains other than ACH5, cna transcription was temporally regulated, with cna mRNA levels being highest in cells taken from exponentially growing cultures and falling to almost undetectable levels as cultures entered the post-exponential growth phase. The CBC was also highest with cells taken from exponentially growing cultures. Mutation of agr resulted in a slight increase in cna transcription and a corresponding increase in CBC during the exponential growth phase but did not affect the temporal pattern of cna transcription. Mutation of sar resulted in a more dramatic increase in CBC and a delay in the post-exponential-phase repression of cna transcription. Mutation of both sar and agr had an additive effect on both CBC and cna transcription. We conclude that (i) cna encodes the primary collagen-binding adhesin in S. aureus, (ii) sar is the primary regulatory element controlling expression of cna, and (iii) the regulatory effects of sar and agr on cna transcription are independent of the interaction between sar and agr.


* Corresponding author. Mailing address: Department of Microbiology and Immunology, Slot 511, University of Arkansas for Medical Sciences, 4301 W. Markham, Little Rock, AR 72205. Phone: (501) 686-7958. Fax: (501) 686-5359. E-mail: smeltzermarks{at}exchange.uams.edu.


Infect Immun, July 1998, p. 3170-3178, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Ymele-Leki, P., Ross, J. M. (2007). Erosion from Staphylococcus aureus Biofilms Grown under Physiologically Relevant Fluid Shear Forces Yields Bacterial Cells with Reduced Avidity to Collagen. Appl. Environ. Microbiol. 73: 1834-1841 [Abstract] [Full Text]  
  • Procopio Evagrio George, N., Wei, Q., Kyun Shin, P., Konstantopoulos, K., Ross, J. M. (2006). Staphylococcus aureus Adhesion via Spa, ClfA, and SdrCDE to Immobilized Platelets Demonstrates Shear-Dependent Behavior. Arterioscler. Thromb. Vasc. Bio. 26: 2394-2400 [Abstract] [Full Text]  
  • Nallapareddy, S. R., Murray, B. E. (2006). Ligand-Signaled Upregulation of Enterococcus faecalis ace Transcription, a Mechanism for Modulating Host-E. faecalis Interaction. Infect. Immun. 74: 4982-4989 [Abstract] [Full Text]  
  • Sambanthamoorthy, K., Smeltzer, M. S., Elasri, M. O. (2006). Identification and characterization of msa (SA1233), a gene involved in expression of SarA and several virulence factors in Staphylococcus aureus.. Microbiology 152: 2559-2572 [Abstract] [Full Text]  
  • Sakinc, T., Kleine, B., Gatermann, S. G. (2006). SdrI, a Serine-Aspartate Repeat Protein Identified in Staphylococcus saprophyticus Strain 7108, Is a Collagen-Binding Protein.. Infect. Immun. 74: 4615-4623 [Abstract] [Full Text]  
  • Sakinc, T., Woznowski, M., Ebsen, M., Gatermann, S. G. (2005). The Surface-Associated Protein of Staphylococcus saprophyticus Is a Lipase. Infect. Immun. 73: 6419-6428 [Abstract] [Full Text]  
  • Cassat, J. E., Dunman, P. M., McAleese, F., Murphy, E., Projan, S. J., Smeltzer, M. S. (2005). Comparative Genomics of Staphylococcus aureus Musculoskeletal Isolates. J. Bacteriol. 187: 576-592 [Abstract] [Full Text]  
  • Park, C. H., Lee, S. J., Lee, S. G., Lee, W. S., Byun, S. M. (2004). Hetero- and Autoprocessing of the Extracellular Metalloprotease (Mpr) in Bacillus subtilis. J. Bacteriol. 186: 6457-6464 [Abstract] [Full Text]  
  • Shaw, L., Golonka, E., Potempa, J., Foster, S. J. (2004). The role and regulation of the extracellular proteases of Staphylococcus aureus. Microbiology 150: 217-228 [Abstract] [Full Text]  
  • Sterba, K. M., Mackintosh, S. G., Blevins, J. S., Hurlburt, B. K., Smeltzer, M. S. (2003). Characterization of Staphylococcus aureus SarA Binding Sites. J. Bacteriol. 185: 4410-4417 [Abstract] [Full Text]  
  • Beenken, K. E., Blevins, J. S., Smeltzer, M. S. (2003). Mutation of sarA in Staphylococcus aureus Limits Biofilm Formation. Infect. Immun. 71: 4206-4211 [Abstract] [Full Text]  
  • Blevins, J. S., Elasri, M. O., Allmendinger, S. D., Beenken, K. E., Skinner, R. A., Thomas, J. R., Smeltzer, M. S. (2003). Role of sarA in the Pathogenesis of Staphylococcus aureus Musculoskeletal Infection. Infect. Immun. 71: 516-523 [Abstract] [Full Text]  
  • Cucarella, C., Tormo, M. A., Knecht, E., Amorena, B., Lasa, I., Foster, T. J., Penades, J. R. (2002). Expression of the Biofilm-Associated Protein Interferes with Host Protein Receptors of Staphylococcus aureus and Alters the Infective Process. Infect. Immun. 70: 3180-3186 [Abstract] [Full Text]  
  • Luong, T., Sau, S., Gomez, M., Lee, J. C., Lee, C. Y. (2002). Regulation of Staphylococcus aureus Capsular Polysaccharide Expression by agr and sarA. Infect. Immun. 70: 444-450 [Abstract] [Full Text]  
  • Blevins, J. S., Beenken, K. E., Elasri, M. O., Hurlburt, B. K., Smeltzer, M. S. (2002). Strain-Dependent Differences in the Regulatory Roles of sarA and agr in Staphylococcus aureus. Infect. Immun. 70: 470-480 [Abstract] [Full Text]  
  • Rice, K., Peralta, R., Bast, D., de Azavedo, J., McGavin, M. J. (2001). Description of Staphylococcus Serine Protease (ssp) Operon in Staphylococcus aureus and Nonpolar Inactivation of sspA-Encoded Serine Protease. Infect. Immun. 69: 159-169 [Abstract] [Full Text]  
  • Pohlmann-Dietze, P., Ulrich, M., Kiser, K. B., Doring, G., Lee, J. C., Fournier, J.-M., Botzenhart, K., Wolz, C. (2000). Adherence of Staphylococcus aureus to Endothelial Cells: Influence of Capsular Polysaccharide, Global Regulator agr, and Bacterial Growth Phase. Infect. Immun. 68: 4865-4871 [Abstract] [Full Text]  
  • Nallapareddy, S. R., Singh, K. V., Duh, R.-W., Weinstock, G. M., Murray, B. E. (2000). Diversity of ace, a Gene Encoding a Microbial Surface Component Recognizing Adhesive Matrix Molecules, from Different Strains of Enterococcus faecalis and Evidence for Production of Ace during Human Infections. Infect. Immun. 68: 5210-5217 [Abstract] [Full Text]  
  • Fujimoto, D. F., Brunskill, E. W., Bayles, K. W. (2000). Analysis of Genetic Elements Controlling Staphylococcus aureus lrgAB Expression: Potential Role of DNA Topology in SarA Regulation. J. Bacteriol. 182: 4822-4828 [Abstract] [Full Text]  
  • Hartford, O., McDevitt, D., Foster, T. J. (1999). Matrix-binding proteins of Staphylococcus aureus: functional analysis of mutant and hybrid molecules. Microbiology 145: 2497-2505 [Abstract] [Full Text]  
  • Snodgrass, J. L., Mohamed, N., Ross, J. M., Sau, S., Lee, C. Y., Smeltzer, M. S. (1999). Functional Analysis of the Staphylococcus aureus Collagen Adhesin B Domain. Infect. Immun. 67: 3952-3959 [Abstract] [Full Text]  
  • Peacock, S. J., Lina, G., Etienne, J., Foster, T. J. (1999). Staphylococcus schleiferi subsp. schleiferi Expresses a Fibronectin-Binding Protein. Infect. Immun. 67: 4272-4275 [Abstract] [Full Text]  
  • Dominiecki, M. E., Weiss, J. (1999). Antibacterial Action of Extracellular Mammalian Group IIA Phospholipase A2 against Grossly Clumped Staphylococcus aureus. Infect. Immun. 67: 2299-2305 [Abstract] [Full Text]  
  • Ouyang, S., Sau, S., Lee, C. Y. (1999). Promoter Analysis of the cap8 Operon, Involved in Type 8 Capsular Polysaccharide Production in Staphylococcus aureus. J. Bacteriol. 181: 2492-2500 [Abstract] [Full Text]  
  • Haziot, A., Hijiya, N., Schultz, K., Zhang, F., Gangloff, S. C., Goyert, S. M. (1999). CD14 Plays No Major Role in Shock Induced by Staphylococcus aureus but Down-Regulates TNF-{alpha} Production. J. Immunol. 162: 4801-4805 [Abstract] [Full Text]  
  • McAleese, F. M., Walsh, E. J., Sieprawska, M., Potempa, J., Foster, T. J. (2001). Loss of Clumping Factor B Fibrinogen Binding Activity by Staphylococcus aureus Involves Cessation of Transcription, Shedding and Cleavage by Metalloprotease. J. Biol. Chem. 276: 29969-29978 [Abstract] [Full Text]