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Infect Immun, July 1998, p. 3208-3217, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Salmonella typhimurium AhpC Polypeptide Is Not Essential for Virulence in BALB/c Mice but Is Recognized as an Antigen during Infection

Patrick D. Taylor, Christopher J. Inchley, and Maurice P. Gallagher*

Division of Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, United Kingdom

Received 29 October 1997/Returned for modification 9 February 1998/Accepted 11 April 1998

The OxyR regulon is known to mediate protection against oxidizing agents in Salmonella typhimurium. We reported previously that ahp, one of the OxyR-regulated loci, is induced during macrophage interaction (K. P. Francis, P. D. Taylor, C. J. Inchley, and M. P. Gallagher, J. Bacteriol. 179:4046-4048, 1997). We now report on the effects of disrupting ahp or oxyR on virulence in a BALB/c mouse model. Surprisingly, insertion of a Mudlux derivative within ahpC was found to result in attenuation, while irreversible inactivation of the locus through insertion of a cml cassette did not. An SL1344 derivative carrying an oxyR::kan disruption was also found to be as virulent as the parental strain. Moreover, both cell-mediated and humoral responses to AhpC were found to develop during the course of infection, probably through T-helper-cell (type I) activation. These results indicate that, although not essential for virulence, AhpC is expressed by S. typhimurium during infection of BALB/c mice and constitutes a target for the immune system.

* Corresponding author. Mailing address: Institute of Cell and Molecular Biology, Division of Biology, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, Scotland, United Kingdom. Phone: 44 131 650 5409. Fax: 44 131 650 8650. E-mail: mp.gallagher{at}ed.ac.uk.

Infect Immun, July 1998, p. 3208-3217, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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