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Infect Immun, July 1998, p. 3270-3278, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Protective Roles of gamma delta T Cells and Interleukin-15 in Escherichia coli Infection in Mice

M. Takano,1,2 H. Nishimura,1 Y. Kimura,1 Y. Mokuno,1,2 J. Washizu,1,2 S. Itohara,3 Y. Nimura,2 and Y. Yoshikai1,*

Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control,1 and First Department of Surgery,2 Nagoya University School of Medicine, Nagoya, and Institute for Virus Research, Kyoto University, Kyoto,3 Japan

Received 12 January 1998/Returned for modification 11 February 1998/Accepted 15 April 1998

The number of gamma delta T cells in the peritoneal cavity was increased after an intraperitoneal (i.p.) infection with Escherichia coli in lipopolysaccharide (LPS)-responsive C3H/HeN mice but not in LPS-hyporesponsive C3H/HeJ mice. The gamma delta T cells preferentially expressed invariant Vgamma 6 and Vdelta 1 chains and proliferated to produce a large amount of gamma interferon in the presence of LPS. Mice depleted of gamma delta T cells by T-cell receptor delta  gene mutation showed impaired resistance against E. coli as assessed by bacterial growth. Macrophages from C3H/HeN mice infected with E. coli expressed higher levels of interleukin-15 (IL-15) mRNA than those from the infected C3H/HeJ mice. Administration of anti-IL-15 monoclonal antibody inhibited, albeit partially, the appearance of gamma delta T cells in C3H/HeN mice after E. coli infection and diminished the host defense against the infection. These results suggest that LPS-stimulated gamma delta T cells play an important role in the host defense against E. coli infection and that IL-15 may be partly involved in the protection via an increase in the gamma delta T cells.

* Corresponding author. Mailing address: Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsuramai-cho, Showa-ku, Nagoya 466, Japan. Phone: 81-52-744-2446. Fax: 81-52-744-2449. E-mail: yyoshika{at}tsuru.med.nagoya.u-ac.jp.

Infect Immun, July 1998, p. 3270-3278, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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