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Infect Immun, July 1998, p. 3423-3428, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Immunogenicity of Four Plasmodium falciparum Preerythrocytic Antigens in Aotus lemurinus Monkeys

Blanca Liliana Perlaza,^1, Myriam Arévalo-Herrera,¹ Karima Brahimi,² Gustavo Quintero,¹ Julio Cesar Palomino,¹ Hélène Gras-Masse,³ André Tartar,³ Pierre Druilhe,^2,* and Sócrates Herrera¹

Instituto de Inmunologia, Universidad del Valle, AA 2188 Cali, Colombia,¹ and Bio-Medical Parasitology Laboratory, Pasteur Institute, Paris,² and Pasteur Institute, Lille,³ France

Received 6 October 1997/Returned for modification 25 November 1997/Accepted 20 April 1998

Aotus lemurinus monkeys were immunized with pools of either lipid-tailed peptides injected in PBS or peptides in Montanide ISA-51, all derived from four Plasmodium falciparum pre-erythrocytic antigens, namely, LSA1, LSA3, SALSA, and STARP. These formulations were well tolerated. Their immunogenicity was demonstrated by the induction of both B- and T-cell responses to most of the peptides studied (of the 12, 10 induced antibody production, 9 induced T-cell proliferative responses, and all 12 induced gamma interferon secretion). Immune responses proved to be long lasting, since some were still detectable 210 days after immunization. Of particular importance is the fact that B- and T-cell responses elicited in this way by synthetic peptides were specific for native parasite proteins on P. falciparum sporozoites and liver stage parasites.

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^* Corresponding author. Mailing address: Laboratoire de Parasitologie Bio-Médicale Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris, Cedex 15, France. Phone: 33 1 45 68 85 78. Fax: 33 1 45 68 86 40. E-mail: druilhe{at}pasteur.fr.

Present address: Laboratoire de Parasitologie Bio-Médicale, Institut Pasteur, 75724 Paris, Cedex 15, France.

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Infect Immun, July 1998, p. 3423-3428, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

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